Waters, 186009164, XBridge Protein BEH SEC Column, 200Å, 2.5 µm, 7.8 mm X 300 mm, 1/pk

XBridge Protein BEH SEC Column, 200Å, 2.5 µm, 7.8 mm X 300 mm Columns, containing 2.5 µm particles are based on the same Waters Ethylene Bridged Hybrid (BEH)-based particle technology and diol-bonded surface coating as used in our successful line of HPLC-based SEC columns. They are best suited to separate proteins ranging from 10,000 to 450,000 Daltons. Each batch is specifically QC tested using Waters BEH200 SEC Protein Standard (p/n: 186006518) to help ensure consistent performance for challenging size-based protein separations. Note: This p/n: is also available with one vial the BEH200 SEC Protein Standard at no additional cost (p/n: 176004327). Suggested Use: Gel Filtration, Size Exclusion Chromatography, Aggregate, Monomer and Fragment analysis

Specifications
Chemistry: SEC
Separation Mode: Gel Filtration (aqueous)
Particle Substrate: Hybrid
pH Range Min: 2.5 pH
pH Range Max: 8 pH
Temperature Limits: 60 C
Maximum Pressure: 6,500 psi (448 Bar)
Endcapped: No
Bonding Technology: Diol (OH)
Silanol Activity: Low
Molecular Weight Range Min: 10000
Molecular Weight Range Max: 450000
Particle Size: 2.5 µm
Endfitting Type: Parker-style
Pore Size: 200 Å
QC Tested: Protein
Format: Online Column
Packing Solvent: 20% methanol in water
Surface Area: 220
System: UHPLC, UPLC, HPLC
Particle Technology: BEH
Technique: LC
USP Classification: L20, L33, L59
Inner Diameter: 7.8 mm
Length: 300 mm
Carbon Load: 12 %
eCord: No
UNSPSC: 41115711
Application: Protein
Brand: XBridge
Product Type: Columns
Units per Package: 1 pk

Additional Information
XBridge Protein BEH SEC Column, 200Å, 2.5 µm, 7.8 mm X 300 mm, 1/pk Invest in the XBridge Protein BEH SEC, 200 Column, which features 2.5um particles of Waters' Ethylene Bridged Hybrid (BEH)-based particle technology and diol-bonded surface coating used in our successful series of HPLC-based SEC columns. With the XBridge Protein BEH SEC Column, you can easily design and transfer SEC procedures based on laboratory apparatus. The lab equipment also allows you to create methods based on the resolution of required protein components and sample throughput requirements. The XBridge Protein BEH SEC Columns were developed specifically for size-exclusion chromatography of proteins to complement Waters' existing line of smaller particle size UPLC-based SEC columns. The XBridge Protein BEH SEC Column offers a longer column life than silica-based SEC columns because it has fewer unwanted protein-column interactions. The lab equipment also provides better throughput and delivers HPLC-based SEC resolution of proteins from 10,000 to 450,000 Daltons. The flow and pressure tolerance of these diol-bonded XBridge Protein BEH SEC Columns allows for enhanced sample throughput on HPLC equipment. They deliver exceptional stability, performance, and lifetime by combining BEH base particle technology with new diol bonding techniques. The XBridge Protein BEH SEC Column, like all lab equipment developed and delivered by Waters, ensures consistent and reliable performance because it is manufactured in cGMP, ISO9001, and ISO13485 certified facilities. Comprehensive testing is also performed at each stage, from packing materials to post-production, to ensure unrivaled column consistency and your confidence in validation techniques. When compared to silica-based SEC columns, the XBridge Protein BEH SEC Column has fewer unwanted protein-column interactions. This allows the column to have a long service life. The diol-bonded XBridge Protein BEH SEC Column allows for higher sample throughput on HPLC systems while providing for flow and pressure tolerance. Column stability, performance, and lifetime can all be achieved by combining BEH base particle technology with new diol bonding techniques. We recommend that you also explore and invest in the BEH200 SEC Protein Standard Mix. The Standard Mix is a 5 component protein mixture (Thyroglobulin, IgG, BSA, Myoglobin, Uracil) used to benchmark analytical columns.

FAQ
Is There An Operating pH Limit For The XBridge Protein BEH SEC Column? The XBridge Protein BEH SEC Column listed here performs well within the operating pH range of 2.5-8.