Abcam, ab1, Anti-HIF-1 alpha antibody [H1alpha67]

Size: 100µg
Anti-HIF-1 alpha antibody [H1alpha67] (ab1) is a mouse monoclonal antibody detecting HIF-1 alpha in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, ICC/IF . Suitable for Human . - Clone H1alpha67 is the most cited clone to HIF-1 alpha - KO validated for confirmed specificity - Over 340 publications - Trusted since 1998
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:H1alpha67,
Isotype:IgG2b,
Carrier free:No,
Reacts with:Human,
Applications:WB, ICC/IF, Flow Cyt (Intra), IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:For WB, we recommend using positive control samples such as DFO or CoCl2 treated nulcear cell lysates such as ab180880. Ensure cell lysis occurs quickly (within 2 mins) if removed from hypoxia. Loading a high amount of sample (>50 µg) and addition of protease inhibitors (e.g. ab65621) may also enhance detection.

Product details:
Anti-HIF-1 alpha antibody [H1alpha67] (ab1) is a House Mouse Monoclonal antibody and is validated for use in Flow Cyt (Intra), ICC/IF, IP, WB.
Anti-HIF-1 alpha antibody [H1alpha67] (ab1) has been cited over 343 times in peer reviewed journals and is trusted by the scientific community.
Abcams high quality validation processes ensure Anti-HIF-1 alpha antibody [H1alpha67] (ab1) has high sensitivity and specificity.
The specificity of Anti-HIF-1 alpha antibody [H1alpha67] (ab1) has been confirmed by testing in knockout samples.
Anti-HIF-1 alpha antibody [H1alpha67] (ab1) has 41 independent reviews from customers.
Anti-HIF-1 alpha antibody [H1alpha67] (ab1) specifically detects HIF-1 alpha (UniProt ID: Q16665; Molecular weight: 93kDa) and is sold in 100 ug selling sizes.
Antibody clone H1alpha67 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 647 (
ab23848
HIF-1 alpha, also known as hypoxia-inducible factor 1-alpha (HIF1A), is a critical regulator of cellular response to low oxygen levels. HIF1A is a transcription factor commonly referred to as a "master regulator of the hypoxic response" for its central role in the regulation of cellular adaptations to hypoxia. Hypoxia contributes to the pathophysiology of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. A highly specific HIF-1 alpha antibody, essential for studying hypoxia-inducible factors and oxygen homeostasis. This antibody is crucial in tumor hypoxia research, particularly in understanding cancer progression and angiogenesis. It is widely used in studies of metastasis and HIF-1 alpha inhibitors. The HIF1A molecular weight is approximately 120 kDa, and detecting it accurately is essential for understanding its role in the tumor microenvironment. Elevated HIF-1 alpha levels are linked to tumor progression, angiogenesis, and metastasis. Monitoring HIF-1 alpha can provide valuable insights into cancer biology and potential therapeutic targets.
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 6.97% L-Arginine, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HIF-1 alpha also known as hypoxia-inducible factor 1-alpha is a transcription factor critical in cellular response to low oxygen levels. Its molecular weight usually ranges from 93 to 120 kDa. You can find HIF-1 alpha expressed in tissues throughout the body but its expression significantly increases under hypoxic conditions. Researchers often use the HIF-1a ELISA to measure its expression levels. HIF-1 alpha forms a complex with other proteins to perform its functions effectively.
Biological function summary
HIF-1 alpha regulates gene expression in response to hypoxic conditions in cells. It forms a complex with HIF-1 beta to activate transcription of various genes involved in energy metabolism angiogenesis and erythropoiesis. HIF-1 alpha enables cells to adapt to reduced oxygen availability allowing for cellular survival and function under stress. It plays an important role in promoting the expression of genes like VEGF and EPO which are important for vascular and red blood cell development respectively.
Pathways
HIF-1 alpha plays an integral role in the hypoxia signaling pathway and the glycolytic pathway. In the hypoxia signaling pathway HIF-1 alpha partners with VHL (Von Hippel-Lindau) protein that regulates its degradation under normal oxygen conditions. When oxygen levels drop HIF-1 alpha avoids degradation stabilizes and translocates into the nucleus to initiate transcription of hypoxia-responsive genes. The glycolytic pathway involvement highlights its function in adapting energy production under hypoxic conditions through collaboration with enzymes and transporters associated with glycolysis.
HIF-1 alpha has been implicated in cancer and ischemic diseases. Its role in promoting angiogenesis and metabolic adaptation makes it a contributor to tumor growth and survival collaborating with oncogenes such as c-Myc. In ischemic diseases like stroke or myocardial infarction HIF-1 alpha's ability to induce protective responses can mitigate tissue damage through regulation of survival pathways. Understanding these interactions helps in the development of therapeutic strategies targeting HIF-1 alpha in disease contexts.