New England Biolabs (NEB) — Enzymes & Reagents for Life Science Research
Founded in 1974 by scientists at Harvard Medical School, New England Biolabs (NEB®) is a world leader in the discovery and production of enzymes for molecular biology research. Headquartered in Ipswich, Massachusetts, NEB operates as a privately held company with subsidiaries across ten countries, including the US, UK, Germany, France, Canada, China, Japan, Singapore, Australia, and South Korea.
NEB's catalog spans restriction enzymes, high-fidelity polymerases, DNA and RNA purification kits, NGS library preparation reagents, epigenetics tools, and gene assembly systems. The company is unique in that it funds an extensive internal research program entirely from its own profits — meaning every product is developed, tested, and refined by working scientists. NEB's Chief Scientific Officer, Sir Richard Roberts, shared the 1993 Nobel Prize in Physiology or Medicine for the discovery of RNA splicing, a distinction that reflects the depth of scientific expertise behind NEB products.
Today, NEB serves research labs across academia, biotechnology, pharmaceuticals, and molecular diagnostics worldwide.
NEB Product Lines: From Restriction Enzymes to NGS Library Prep
NEB offers one of the broadest enzyme and reagent catalogs in the life sciences industry. Below is an overview of its major product lines:
Restriction Enzymes NEB produces over 260 restriction enzymes — the largest selection of any supplier — including both recombinant and native formats. The HF® (High-Fidelity) enzyme series delivers reduced star activity and enhanced cut-to-cut consistency, making them the preferred choice for cloning-critical applications.
PCR & High-Fidelity Polymerases NEB's polymerase range includes Phusion™ High-Fidelity DNA Polymerase, one of the most widely cited polymerases in molecular biology, alongside the Q5® High-Fidelity DNA Polymerase for applications requiring the lowest possible error rates. Both are well-suited for cloning, library preparation, and amplification of difficult templates.
NEBNext® — NGS Library Prep The NEBNext® product line covers end-to-end library preparation workflows for Illumina, Oxford Nanopore (ONT), and PacBio platforms. Kits address DNA, RNA, methylation sequencing, and targeted enrichment applications. NEBNext is the NGS library prep system of choice in sequencing cores and clinical research settings globally.
NEBuilder® — DNA Assembly NEBuilder® HiFi DNA Assembly Master Mix enables seamless, scarless assembly of multiple DNA fragments in a single isothermal reaction. It is widely used for synthetic biology, pathway construction, and complex cloning workflows where traditional restriction-ligation approaches are inefficient.
Monarch® — Nucleic Acid Purification The Monarch® purification kit series covers genomic DNA, PCR product cleanup, plasmid miniprep, RNA miniprep, and total RNA isolation. Monarch kits are designed for minimal plasticware and environmentally conscious lab workflows without compromising yield or purity.
Luna® — qPCR & RT-qPCR Luna® Universal qPCR and RT-qPCR Master Mixes are optimized for broad primer compatibility, high sensitivity, and consistent performance across real-time PCR platforms. The Luna line is a direct, high-performance alternative to established qPCR reagent brands.
EpiMark® — Epigenetics Research The EpiMark® series provides validated reagents for studying DNA methylation, histone modification, and chromatin accessibility. NEB's epigenetics tools are designed to work within both established ChIP workflows and next-generation approaches such as EM-seq™ for 5mC/5hmC detection.
Competent Cells & Cloning Tools NEB supplies a comprehensive range of competent E. coli strains — from general-purpose cloning strains such as DH5α to specialized cells for unstable inserts, expression, and protein production. Companion cloning reagents include ligases, phosphatases, and kinases.
Frequently Ordered NEB Products & Catalog Numbers
The table below covers the most commonly ordered NEB catalog items sourced through Iright. All products are available for quote on request.
| Cat. No. | Product Name | Primary Application |
|---|---|---|
| R0101S/L | EcoRI | Restriction digestion |
| R0136S/L | HindIII | Restriction digestion |
| R3136S/L | HindIII-HF® | High-fidelity restriction digestion |
| R3101S/L | EcoRI-HF® | High-fidelity restriction digestion |
| M0202S/L/T | T4 DNA Ligase | DNA ligation |
| M0210S/L | T4 Polynucleotide Kinase (T4 PNK) | 5′ phosphorylation / dephosphorylation |
| M0530S/L | Phusion™ High-Fidelity DNA Polymerase | High-fidelity PCR, cloning |
| M0491S/L | Q5® High-Fidelity DNA Polymerase | Ultra-high-fidelity PCR |
| E2621S/L | NEBuilder® HiFi DNA Assembly Master Mix | Multi-fragment DNA assembly |
| E6440S/L | NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® | NGS library preparation |
| E7490S/L | NEBNext® Ultra™ II FS DNA Library Prep Kit | Fragmentation-based NGS library prep |
| T4050S/L | Luna® Universal qPCR Master Mix | Real-time PCR |
| T3010S/L | Luna® Universal One-Step RT-qPCR Kit | RNA quantification / RT-qPCR |
| T1030S/L | Monarch® Plasmid Miniprep Kit | Plasmid DNA purification |
| T3006S/L | Monarch® PCR & DNA Cleanup Kit | PCR product cleanup |
| T2010S/L | Monarch® RNA Miniprep Kit | Total RNA extraction |
| P7712S/L | DH5α Competent E. coli | General cloning |
S = 20–50 rxn; L = 100–500 rxn; T = bulk/trial size. Contact us to confirm current pack sizes and pricing.
How to Source NEB Products Through Iright
Iright is a global lab supply sourcing platform serving research institutions, biotech companies, and procurement teams across multiple regions. Whether you need a single NEB kit or a standing order for high-volume reagents, Iright offers a reliable alternative sourcing channel with competitive pricing and dedicated account support.
- Authentic products — all NEB items sourced through verified supply channels with full documentation
- Global reach — serving labs across Europe, the Middle East, Latin America, and Asia
- Consolidated ordering — combine NEB with other brands in a single purchase order
- Responsive quoting — most requests receive pricing and availability confirmation within 24 hours
- Backup sourcing support — a dependable secondary channel for labs with existing NEB supply relationships