Abcam, ab100502, Human DKK3 ELISA Kit (Dickkopf-3)

Size: 1 x 96Tests
Human DKK3 ELISA Kit (Dickkopf-3) is a Sandwich (quantitative) ELISA for the measurement of Human DKK3 (Dickkopf-3) in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:< 3 pg/mL,
Range:2.74 - 2000 pg/mL,
Assay Platform:Microplate

Product details:
Abcam's DKK3 (Dickkopf-3) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an
in vitro
enzyme-linked immunosorbent assay for the quantitative measurement of Human DKK3 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human DKK3 coated on a 96-well plate. Standards and samples are pipetted into the wells and DKK3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human DKK3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DKK3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimisation may be required with urine samples.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
DKK3 also known as Dickkopf-related protein 3 or Dickkopf-3 is a member of the Dickkopf protein family. This secreted protein has a molecular mass of approximately 38 kDa. DKK3 is expressed in multiple tissues including heart brain and kidney. It plays a role in modulating signaling pathways by acting as a Wnt signaling pathway antagonist. It's important in the regulation of developmental processes and is often investigated in various cellular contexts.
Biological function summary
DKK3 functions as an inhibitor of the Wnt/beta-catenin signaling pathway. It is not known to be a part of a larger protein complex but likely interacts with specific receptors and co-receptors at the cell surface to modulate signaling events. This regulation influences cell fate decisions proliferation and differentiation making DKK3 an essential player in cellular development and homeostasis.
Pathways
DKK3 significantly impacts the Wnt and TGF-beta signaling pathways. As an inhibitor of Wnt signaling DKK3 prevents the stabilization and accumulation of beta-catenin in the cytoplasm thereby affecting gene transcription in the nucleus. DKK3's interaction with TGF-beta proteins also influences cellular responses to growth factors and cytokines facilitating communication within the cell and its environment.
Alterations in DKK3 expression have links to cancers and cardiovascular diseases. In cancer DKK3 often exhibits reduced expression affecting pathways that regulate tumor growth and metastasis. In cardiovascular disorders DKK3 interacts with proteins such as Frizzled and LRP5/6 which relate to disease pathogenesis. Monitoring DKK3 using assays like DKK3 ELISA can help in understanding its role in disease progression and could serve as a potential biomarker or therapeutic target.