Abcam, ab100503, Human DKK4 ELISA Kit (Dickkopf-4)

Size: 1 x 96Tests
Human DKK4 ELISA Kit (Dickkopf-4) is a Sandwich (quantitative) ELISA for the measurement of Human DKK4 (Dickkopf-4) in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:< 10 pg/mL,
Range:13.72 - 10000 pg/mL,
Assay Platform:Microplate

Product details:
Abcam's DKK4 (Dickkopf-4) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an
in vitro
enzyme-linked immunosorbent assay for the quantitative measurement of Human DKK4 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human DKK4 coated on a 96-well plate. Standards and samples are pipetted into the wells and DKK4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human DKK4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DKK4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimisation may be required with urine samples.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Dickkopf-related protein 4 (DKK4) acts as an inhibitor of the Wnt signaling pathway which affects cell fate proliferation and migration. Known aliases for DKK4 include DKK-4 and DKK-4 protein. It possesses a molecular mass of around 27 kDa. DKK4 mainly expresses in the skin gastrointestinal tract and placenta indicating roles in these tissues and systems. Its presence suggests involvement in processes related to epithelial and stromal interactions.
Biological function summary
DKK4 participates in the regulation of embryonic development and tissue homeostasis. It functions as a part of the larger Dickkopf protein family which modulates Wnt signaling by binding to LRP5/6 co-receptors. Through this interaction DKK4 manages the balance between cell growth and apoptosis important in both early development and adult tissue maintenance. DKK4 presence is often necessary to modulate signals that govern differentiation and proliferation in complex tissue systems.
Pathways
DKK4 predominantly interacts within the Wnt signaling pathway. This pathway is critical for regulating gene expression involved in stem cell maintenance and differentiation. DKK4 acts by interacting with proteins such as LRP6 and Kremen thereby inhibiting Wnt-induced processes. Through modulation of these factors DKK4 ensures the fine-tuning of signaling cascades necessary for maintaining cellular equilibrium under physiological conditions.
Research associates DKK4 with malignant melanoma and colorectal cancer. These associations arise due to DKK4's role in modulating Wnt signaling which can drive oncogenic processes if dysregulated. In melanoma DKK4 can affect tumor cell proliferation by interacting with proteins like beta-catenin. In colorectal cancer abnormal expression of DKK4 might disrupt normal signaling balance further implicating it in tumor growth and progression. These connections highlight DKK4's potential as both a biomarker and a therapeutic target.