Abcam, ab100523, Human Galectin 7 ELISA Kit (GAL7)

Size: 1 x 96Tests
Human Galectin 7 ELISA Kit (GAL7) is a Sandwich (quantitative) ELISA for the measurement of Human Galectin 7 (GAL7) in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:< 20 pg/mL,
Range:13.72 - 10000 pg/mL,
Assay Platform:Microplate

Product details:
Abcam's Galectin 7 (GAL7) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an
in vitro
enzyme-linked immunosorbent assay for the quantitative measurement of Human Galectin 7 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human Galectin 7 coated on a 96-well plate. Standards and samples are pipetted into the wells and Galectin 7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Galectin 7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Galectin 7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Galectin 7 also known as Gal-7 is a member of the galectin family of beta-galactoside-binding proteins. It has a molecular mass of approximately 15 kDa. Structurally Galectin 7 has a single carbohydrate recognition domain (CRD) which allows it to bind glycoconjugates. Galectin 7 expresses in various tissues with high expression levels in the epidermis and in stratified squamous epithelia.
Biological function summary
Galectin 7 plays a role in cell-cell adhesion apoptosis regulation and immune response modulation. It plays a role in the cell's apoptosis machinery and interacts with the cytochrome c release pathway. This protein does not typically form a part of large complexes functioning individually in most biological processes. It can influence keratinocyte migration indicating involvement in wound healing processes.
Pathways
Galectin 7 participates in the apoptosis and cell adhesion pathways. It works alongside proteins such as Bcl-2 and Bax to regulate programmed cell death by promoting apoptosis. Additionally it interacts with matrix metalloproteinases influencing the remodelling of extracellular matrix a critical component of tissue repair and immune response.
Galectin 7 is associated with cancer and psoriasis. In cancer it can act as a pro-apoptotic factor and modulates tumor progression often in concert with p53 a well-known tumor suppressor protein. In psoriasis aberrant expression of Galectin 7 correlates with impaired epidermal function and inflammation suggesting it may alter the disease course by influencing local immune responses and keratinocyte behavior.