Abcam, ab100527, Human TNFRSF18 ELISA Kit (GITR)

Size: 1 x 96Tests
Human TNFRSF18 ELISA Kit (GITR) is a Sandwich (quantitative) ELISA for the measurement of Human TNFRSF18 (GITR) in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:< 15 pg/mL,
Range:13.7 - 10000 pg/mL,
Assay Platform:Microplate

Product details:
Abcam's TNFRSF18 (GITR) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an
in vitro
enzyme-linked immunosorbent assay for the quantitative measurement of Human TNFRSF18 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human TNFRSF18 coated on a 96-well plate. Standards and samples are pipetted into the wells and TNFRSF18 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TNFRSF18 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNFRSF18 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C