Abcam, ab100531, Human HB EGF ELISA Kit

Size: 1 x 96Tests
Human HB EGF ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human HB EGF in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:< 20 pg/mL,
Range:16.38 - 4000 pg/mL,
Assay Platform:Microplate

Product details:
Abcam's HB EGF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an
in vitro
enzyme-linked immunosorbent assay for the quantitative measurement of Human HB EGF in plasma and cell culture supernatants. (Human HB EGF concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for Human HB EGF coated on a 96-well plate. Standards and samples are pipetted into the wells and HB EGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human HB EGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HB EGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect the endogenous Human HB-EGF in normal serum with ab100531, only in serum spiked with Human HB-EGF.
Optimization may be required with urine samples.
The standard is affinity purified.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HB-EGF also known as heparin-binding EGF-like growth factor is a member of the EGF family with an approximate molecular mass of 19-23 kDa. It is a membrane-anchored growth factor that can be cleaved to release a soluble form. HB-EGF expresses in a wide range of tissues including the heart kidneys lungs and the brain. As a growth factor its primary mechanical function involves binding to EGFR (epidermal growth factor receptor) and ERBB4 receptors stimulating cellular proliferation and survival.
Biological function summary
The actions of HB-EGF extend beyond simple growth factor roles. It participates as a modulator in wound healing processes cardiac development and neural tissue maintenance. The protein often functions as part of a signaling complex that involves other growth factors and receptors. Its engagement in biological processes links directly to modifying cellular behavior such as enhancing cell migration and inducing tissue repair mechanisms.
Pathways
HB-EGF fits into critical signaling routes such as the MAPK/ERK and PI3K/Akt pathways. These pathways are important in mediating cell growth survival and differentiation. HB-EGF associates with other proteins in these pathways such as Ras and Akt to facilitate downstream signaling that regulates cellular responses to environmental stimuli. The proper function of these pathways is essential for maintaining cellular homeostasis.
HB-EGF has strong associations with cancer and cardiovascular diseases. In various cancer types overexpression or dysregulation of HB-EGF contributes to tumorigenesis and progression through its interaction with EGFR promoting uncontrolled cellular proliferation. Similarly in cardiovascular diseases HB-EGF influences cardiac hypertrophy and heart failure by interacting with associated proteins such as MMPs (matrix metalloproteinases) which further modulate cardiac remodeling and dysfunction. Understanding these relationships helps in therapeutic targeting for related ailments.