Abcam, ab100739, Mouse RANTES ELISA Kit (CCL5)

Size: 1 x 96Tests
Mouse RANTES ELISA Kit (CCL5) is a sandwich ELISA designed to quantify Mouse RANTES (CCL5) with a sensitivity of 1 pg/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 0.82 - 200 pg/mL
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:< 1 pg/mL,
Range:0.82 - 200 pg/mL,
Assay Platform:Microplate

Product details:
Mouse RANTES ELISA Kit (CCL5) ab100739 is a sandwich ELISA to measure Mouse RANTES (CCL5) in serum, plasma, cell culture supernatant with a sensitivity of 1 pg/ml.
How the assay works
This assay employs an antibody specific for mouse RANTES coated on a 96-well plate. Standards and samples are pipetted into the wells and RANTES present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse RANTES antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RANTES bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Mouse RANTES ELISA Kit (CCL5) ab100739 protocol summary
1. Add standard or sample to each well used. Incubate at room temperature
2. Wash and add prepared biotinylated antibody to each well. Incubate at room temperature.
3. Wash and add prepared streptavidin solution. Incubate at room temperature.
4. Add TMB Development Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450nm immediately.
Get results in 90 minutes with our SimpleStep ELISA
Mouse RANTES ELISA Kit (
ab215537

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C