Abcam, ab100756, Pig TNF alpha ELISA Kit

Size: 1 x 96Tests
Pig TNF alpha ELISA Kit is a sandwich ELISA designed to quantify Pig TNF alpha with a sensitivity of 20 pg/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 0.049 - 10 ng/mL
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Pig,
Assay type:Sandwich (quantitative),
Sensitivity:< 20 pg/mL,
Range:0.049 - 10 ng/mL,
Assay Platform:Microplate

Product details:
Pig TNF alpha ELISA Kit ab100756 is a sandwich ELISA to measure Pig TNF alpha in serum, plasma, cell culture supernatant with a sensitivity of 20 pg/ml.
How the assay works
This assay employs an antibody specific for Pig TNF alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and TNF alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Pig TNF alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNF alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Pig TNF alpha ELISA Kit ab100756 protocol summary
1. Add standard or sample to each well used. Incubate at room temperature
2. Wash and add prepared biotinylated antibody to each well. Incubate at room temperature.
3. Wash and add prepared streptavidin solution. Incubate at room temperature.
4. Add TMB Development Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450nm immediately.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C