Abcam, ab10374, Anti-hnRNP H antibody

Size: 50µg
Rabbit Polyclonal hnRNP H antibody. Suitable for IHC-P, IP, WB, ICC/IF, IHC-Fr and reacts with Human, Mouse samples. Cited in 35 publications. Immunogen corresponding to Synthetic Peptide within Human HNRNPH1 aa 400 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:ICC/IF, IHC-Fr, WB, IP, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human HNRNPH1 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.P31943,
Specificity:The antibody was designed against hnRNPH1 and does not recognize hnRNPH3. The epitope recognized by ab10374 maps to a region between residue 400 and the C-terminus (residue 448) of human Heterogeneous Nuclear Ribonucleoprotein H1 using the numbering given in entry NP_005511.1 (GeneID 3187). This product may cross-react with hnRNPH2.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Antibodies were affinity purified using the peptide immobilized on solid support., Storage buffer-pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The heterogeneous nuclear ribonucleoprotein H (hnRNP H) also sometimes referred to as hnRNP H1 is an RNA-binding protein with a molecular mass of approximately 49 kDa. It belongs to the hnRNP family of proteins which are involved in the processing and transport of pre-mRNA. This protein is expressed in the nucleus of eukaryotic cells and plays a role in RNA stability and metabolism.
Biological function summary
HnRNP H acts as a splicing regulator and is a member of the hnRNP complex. It binds to the RNA cis-elements influencing alternative splicing events. hnRNP H can interact with other RNA-binding proteins and regulatory elements to modulate the inclusion or exclusion of certain exons. This helps in generating different mRNA variants allowing the production of protein diversity from a single gene.
Pathways
HnRNP H participates in the alternative splicing and mRNA processing pathways. The proteins it interacts with such as serine/arginine-rich splicing factor 1 (SRSF1) help facilitate the splicing mechanisms. hnRNP H's ability to influence splicing makes it essential for cellular processes like development and differentiation by producing proteins with varied functionalities.
HnRNP H has connections with cancer and neurodegenerative diseases. Dysregulation of hnRNP H's splicing activity has been linked to cancers like glioblastoma where it impacts the expression of oncogenic proteins. Additionally hnRNP H's role in splicing has associations with conditions like amyotrophic lateral sclerosis (ALS) where proteins such as TAR DNA-binding protein 43 (TDP-43) might also be involved.