Abcam, ab104141, Limonene Mounting Medium

Size: 30mL
Limonene/Organo Mounting Medium (ab104141) is a ready-to-use organic mounting medium compatible with chromogens such as DAB in immunoblotting and IHC applications.
Key facts
Form:LiquidSee storage information,
Storage buffer:Constituents: 75% D-Limonene

Product details:
This mounting medium is made with limonene a natural product from orange peels. It is good for preserving tissues and cell smears that can be dehydrated with organic solvents in Immunohistochemistry (IHC) e.g. DAB and DAB with nickel or cobalt. This Organo mounting medium is also suitable with alkaline phosphatase chromogens. It is an excellent choice for mounting H and E stained slides.
Intended Use
Mounting tissues, cell smears in Immunohistochemistry for chromogens that are resistant to organic solvents e.g. DAB, DAB with cobalt, DAB with Nickel any other chromogens that are resistant to organic solvents dehydration. H & E (hematoxylin and Eosin) staining
Procedure
Dehydrate tissue or cell smear slides with dehydrating agents e.g. 30 to 100% alcohol followed by xylene, toluene or any other dehydrating reagents.
Add 2-3 drops of Organo mounting medium. Apply coverslip carefully, avoiding air bubbles.
Visualize slide under a microscope. The slides can be dried by leaving at room temperature overnight or by heating at 37-40°C for several hours.
Limonene Mounting Medium ab104141 is an organic mounting medium compatible with chromogens such as DAB. Suitable for hematoxylin and eosin (H and E) staining.
This mounting medium is made with limonene, a natural product from orange peels. It is good for preserving tissues and cell smears that can be dehydrated with organic solvents in immunohistochemistry (IHC) e.g. DAB and DAB with nickel or cobalt. It is an excellent choice for mounting H and E stained slides.
Intended Use
Mounting tissues, cell smears in Immunohistochemistry for chromogens that are resistant to organic solvents e.g. DAB, DAB with cobalt, DAB with Nickel any other chromogens that are resistant to organic solvents dehydration. H & E (hematoxylin and Eosin) staining.
Mounting media products
For fluorescent cell and tissue staining, Abcam recommends aqueous, anti-fade Fluorescence Mounting Medium
ab104135
, Mounting Medium with DAPI
ab104139
, and Mounting Medium with PI
ab104129
. For thick sections or tissues containing lots of fat, we recommend Glycerol Mounting Medium with DAPI
ab188804
For chromogenic immunohistochemistry with the organic solvent-soluble substrates AEC or Fast Red, we recommend aqueous Mounting Medium
ab64230
Procedure
Dehydrate tissue or cell smear slides with dehydrating agents e.g. 30 to 100% alcohol followed by xylene, toluene or any other dehydrating reagents.
Add 2-3 drops of mounting medium. Apply coverslip carefully, avoiding air bubbles.
Visualize slide under a microscope. The slides can be dried by leaving at room temperature overnight or by heating at 37-40°C for several hours.

Properties and Storage Information:
Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions-Ambient, Appropriate long-term storage conditions-Ambient