Abcam, ab104870, Anti-H1N1 Influenza A virus Nucleocapsid protein antibody

Size: 200µL
Rabbit Polyclonal NCAP antibody. Suitable for ELISA, WB, ICC/IF and reacts with Influenza A samples. Cited in 16 publications.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Influenza A,
Applications:ICC/IF, WB, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Properties and Storage Information:
Form-Liquid, Purity-Whole antiserum, Storage buffer-Constituents: Whole serum, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The H1N1 Influenza A virus nucleocapsid protein also known as the nucleoprotein (NP) plays a central role in the encapsidation of the viral RNA genome. With a molecular mass of approximately 56 kDa NP forms ribonucleoprotein (RNP) complexes that are essential in the virus's replication and transcription processes. The NP is expressed in the cytoplasm of infected host cells where it binds to viral RNA facilitating the formation of helical RNP structures. These structures are critical for the stability and function of the viral genome.
Biological function summary
The NP participates in the virus life cycle by enabling RNA synthesis and interacting with other viral proteins. As part of the RNP complex it collaborates with the RNA polymerase complex made up of PA PB1 and PB2 proteins. This interaction is essential for the transcription and replication of viral RNA. Additionally NP is known to activate host cell signaling pathways that modulate immune response helping the virus evade immune detection and destruction.
Pathways
The NP protein interacts mainly with the viral replication and transcription mechanisms. The replication-transcription pathway features the synchronization of NP with the RNA polymerase complex ensuring the efficient synthesis of new viral genome copies. The NP also interacts with signaling pathways that involve host proteins like importins which regulate the nuclear import of the RNP complex important for its function.
NP is intimately associated with influenza outbreaks especially those caused by the H1N1 flu virus. This protein's interaction with host immune pathways can alter the immune response contributing to the virus's pathogenicity. Its relation to influenza is also marked by its role in viral replication where it works alongside the M1 matrix protein to export progeny RNPs from the nucleus. Understanding NP is important in the development of flu antibodies as it presents a valuable target for vaccines and antiviral treatments against H1N1 influenza.