Abcam, ab105209, Recombinant human NUAK2 protein

Size: 5µg / 10µg
Recombinant human NUAK2 protein is a Human Full Length protein, in the 1 to 628 aa range, expressed in Baculovirus infected Sf9 cells, with >75%, suitable for SDS-PAGE, WB, FuncS.
Key facts
Purity:>75% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:Tag free,
Applications:FuncS, WB, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The Specific activity of ab105209 was determined to be 80 nmol/min/mg.,
Accession:Q9H093,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.307% Glutathione, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00292% EDTA, 0.00174% PMSF

Product details:
ab204854
(Cdc25C peptide) can be utilized as a substrate for assessing kinase activity

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NUAK2 also known as SNARK or NUAK family kinase 2 is a serine/threonine-protein kinase involved in various cellular processes. With a molecular mass of approximately 74 kDa NUAK2 is expressed in multiple tissues notably in the brain skeletal muscle and heart. This kinase plays an important role in regulating cellular energy balance and responds to metabolic stresses acting as a critical mediator in the cellular response to nutrient availability.
Biological function summary
NUAK2 plays an active role in modulating cellular adhesion migration and proliferation. It is not part of a well-defined complex but interacts with several cellular components to exert its functions. NUAK2 participates in the regulation of the actin cytoskeleton impacting cell structure and movement and is involved in maintaining cellular homeostasis through metabolic regulation. It also influences signaling pathways that regulate cellular growth and survival.
Pathways
NUAK2 plays significant roles in the AMP-activated protein kinase (AMPK) signaling pathway and the insulin signaling pathway. As a downstream effector of these pathways NUAK2 impacts important metabolic processes by regulating energy utilization and promoting cell survival under stress conditions. It relates to proteins such as LKB1 which is an upstream kinase that phosphorylates and activates NUAK2 and AMPK which shares signaling pathways impacting metabolism and cell growth.
Various studies have linked NUAK2 to cancer and metabolic disorders. Overexpression of NUAK2 has been observed in several types of cancer including breast and colorectal cancer where it may contribute to tumorigenic processes by promoting cell proliferation and migration. Additionally NUAK2 is connected to hepatic steatosis influencing lipid accumulation in liver cells. In cancer NUAK2 interacts with proteins like MYPT1 which assists in regulating substrate phosphorylation further contributing to its role in cancer biology.