Abcam, ab106289, Anti-Glutamate Transporter 1 antibody

Size: 200µL
Rabbit Polyclonal EAAT2 antibody. Suitable for WB and reacts with Mouse, Rat samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Rat Slc1a2.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Rat Slc1a2. The exact immunogen used to generate this antibody is proprietary information.P31596

Properties and Storage Information:
Form-Liquid, Purity-Whole antiserum, Storage buffer-Constituents: Whole serum, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Glutamate Transporter 1 (GLT-1) also recognized as Excitatory Amino Acid Transporter 2 (EAAT2) is essential for regulating the levels of glutamate in the central nervous system. This protein features a mass of approximately 70 kDa and is largely expressed in astrocytes within the brain. It operates by transporting glutamate from the extracellular space into the cells utilizing the sodium concentration gradient as a driving force. This active transport mechanism prevents the accumulation of glutamate in the synaptic cleft reducing excitotoxicity which is harmful to neural cells.
Biological function summary
GLT-1 serves to maintain neurotransmitter homeostasis critical for proper synaptic functioning. It is a part of the larger SLC1 (solute carrier family 1) protein family which includes other transporters with similar functions. GLT-1 works closely with other glutamate transporters to help ensure synaptic signalings such as sensory perception and cognition proceed without interruption or damage. By efficiently clearing excessive glutamate from synapses it helps protect neurons from excitotoxic stress which can lead to cell death.
Pathways
This transporter is integral in the glutamatergic signaling pathway which plays an important role in synaptic plasticity and memory formation. GLT-1 closely interacts with proteins involved in metabolic pathways such as the glutamate-glutamine cycle ensuring that glutamate is recycled efficiently without causing cellular stress. It is also linked with EAAT1 another member of the excitatory amino acid transporters working in tandem to modulate the balance of neurotransmitter levels.
Mutations or dysfunctions of GLT-1 have been implicated in neurological conditions such as amyotrophic lateral sclerosis (ALS) and epilepsy. In ALS the downregulation of GLT-1 can lead to insufficient clearance of glutamate contributing to motor neuron degeneration. This protein is additionally associated with EAAT2 which is often studied for its role in preventing excitotoxic damage that might exacerbate these neurologic conditions. Researchers are exploring therapeutics like riluzole to modulate GLT-1 activity and alleviate symptoms related to these diseases.