Abcam, ab106307, Recombinant human VEGF189 protein

Size: 5µg
Recombinant human VEGF189 protein is a Human Full Length protein, in the 27 to 232 aa range, expressed in Escherichia coli, with >98%, < 0.1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>98% SDS-PAGE,
Endotoxin level:< 0.1 EU/µg,
Expression system:Escherichia coli,
Tags:Tag free,
Applications:FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Measured by the stimulation of cell proliferation in human umbilical vein endothelial cells in the range of 2-20 ng/ml.,
Accession:P15692,
Animal free:No,
Carrier free:No,
Species:Human,
Reconstitution:Reconstitute at 50 µg/mL in PBS, 0.1% BSA,
Storage buffer:Constituents: 0.3% Acetic acid

Product details:
Molecular weight:
~42 kDa (Dimer)

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-A few weeks, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
VEGF189 also known as Vascular Endothelial Growth Factor 189 plays key role in angiogenesis. With a mass of approximately 21 kDa VEGF189 belongs to the VEGF family which includes other isoforms like VEGF121 and VEGF165. It shows activity in endothelial cells and exists primarily in heparin-binding form. VEGF189 is expressed prominently in the vascular tissues influencing the growth and maintenance of blood vessels. The protein promotes endothelial cell division and increases vascular permeability.
Biological function summary
VEGF189 regulates physiological processes in the body by influencing angiogenesis and vasculogenesis. It does not function alone but interacts with other isoforms and binds to receptors like VEGFR-1 and VEGFR-2 forming active signaling complexes. Unlike its more soluble relatives VEGF189 stays mostly bound to the extracellular matrix. This binding affects tissue-specific patterns of blood vessel growth making it significant in development and wound healing.
Pathways
VEGF189 sits at a critical intersection within the PI3K/Akt and MAPK signaling pathways. The protein interacts with other molecules such as VEGFR-2 leading to downstream signaling events that promote survival and proliferation of endothelial cells. In these pathways VEGF189 works alongside other growth factors to modulate blood vessel formation. Its precise regulation is essential for maintaining tissue homeostasis and responding to hypoxic conditions.
VEGF189 contributes to pathologies like cancer and diabetic retinopathy through its role in abnormal blood vessel growth. In cancer the protein promotes tumor angiogenesis which aids in tumor enlargement and metastasis. The interaction with proteins like VEGFR-2 becomes significant here as they serve as therapeutic targets to inhibit excessive angiogenesis. In diabetic retinopathy the dysregulation of VEGF189 can lead to formation of fragile blood vessels in the retina risking vision loss.