Abcam, ab108679, Human Prolactin ELISA Kit

Size: 1 x 96Tests
Human Prolactin ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Prolactin in Human in Biofluids samples.
Key facts
Detection method:Colorimetric,
Sample types:Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.25 ng/mL,
Range:5 - 100 ng/mL,
Assay Platform:Microplate

Product details:
Abcam's Prolactin
in vitro
competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Prolactin in Human serum.
Microtiterstrip wells are precoated with inactivated specific Corynebacterium diphtheriae toxin (toxoid) antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-human IgG conjugate is added. This conjugate binds to the captured C. diphtheriae toxin-specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of C. diphtheriae toxin-specific IgG antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Prolactin also known as PRL is a protein hormone with a molecular mass of approximately 23 kDa. It is primarily produced in the anterior pituitary gland but is also found in other tissues such as the placenta mammary glands and immune cells. Mechanically Prolactin functions as a peptide hormone and exerts its effects by binding to the Prolactin receptor a member of the cytokine receptor superfamily. This binding activates the Jak-STAT signaling pathway which is important for mediating the hormone's effects in target cells.
Biological function summary
The functions of this hormone extend beyond its well-known role in lactation. It helps stimulate milk production in the mammary glands by promoting the proliferation and differentiation of mammary epithelial cells. Additionally Prolactin influences reproductive functions metabolism and immune regulation. It is not part of a larger protein complex but interacts with various proteins to perform its functions. Notably it modulates the immune response contributing to tolerance during pregnancy and affecting the balance between pro-inflammatory and anti-inflammatory cytokines.
Pathways
Prolactin plays important roles in the Jak-STAT and MAPK signaling pathways. In these pathways it shares interactions with related proteins such as Growth Hormone and Prolactin Receptor aiding in cell growth differentiation and metabolism regulation. Prolactin's involvement in these pathways highlights its central role in physiological processes linking various hormonal signals to regulate body functions efficiently.
Prolactin's imbalance can lead to conditions such as hyperprolactinemia and prolactinomas both characterized by excessive levels of this hormone in the blood. These conditions are often related to alterations in the pituitary gland function. Prolactin interacts closely with Dopamine a neurotransmitter that inhibits its secretion influencing both its production and release. Dysregulation of this interaction may contribute to the development of these disorders impacting endocrine health and overall well-being.