Abcam, ab108834, Human Factor XI ELISA Kit

Size: 1 x 96Tests
Human Factor XI ELISA Kit is a sandwich ELISA used to quantify Human Factor XI with a sensitivity of 0.75 ng/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide measuring range: 1.563 - 50 ng/mL
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:>= 0.75 ng/mL,
Range:1.563 - 50 ng/mL,
Assay time:4h,
Assay Platform:Microplate

Product details:
Human Factor XI ELISA Kit is a sandwich ELISA kit to measure Human Factor XI in plasma, serum with a sensitivity of 0.75 ng/mL.
How the assay works
A Factor XI specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor XI specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor XI captured in plate.
Assay Specificity
No significant cross-reactivity observed with human factor I (fibrinogen), factor II (prothrombin), factor III (tissue factor), factor V, factor VII, factor VIII, factor IX, factor X, factor XII, and factor XIII, factor XIV (protein C), and VWF proteins
Human Factor XI ELISA Kit ab108834 protocol summary:
1. Add standard or sample to each well used. Incubate at room temperature
2. Wash and add prepared biotin antibody to each well. Incubate at room temperature.
3. Wash and add prepared StreptavidinPeroxidase Conjugate. Incubate at room temperature.
4. Add Chromogen Substrate to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read immediately.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Factor XI (FXI) also known as Factor 11 or Factor Eleven is an important protein in the human body involved in blood coagulation. It has a molecular mass of approximately 160 kDa and is primarily expressed in the liver. Factor XI circulates in the blood as a zymogen an inactive enzyme precursor until it is activated during the coagulation process. Its activation is a critical step in the chain of events leading to blood clot formation. FXI can be measured using assays such as ELISA and it is often used in laboratory settings to study coagulation mechanics.
Biological function summary
FXI plays a significant role in the intrinsic pathway of the coagulation cascade. It forms a complex with high-molecular-weight kininogen and prekallikrein on the surface of activated platelets ensuring that the coagulation process proceeds efficiently. Upon activation by Factor XIIa FXI itself becomes an active serine protease known as FXIa which then converts Factor IX into its active form Factor IXa. This step highlights the importance of FXI in amplifying the coagulation process ensuring rapid and effective hemostasis.
Pathways
Factor XI is deeply integrated into the intrinsic coagulation cascade. It works alongside other proteins such as Factor XII and Factor IX to facilitate the formation of a blood clot. The intrinsic pathway is essential for maintaining hemostasis particularly in response to internal injuries within the blood vessels. Its interaction with these proteins allows for a powerful amplification of the clotting signal ensuring a robust clot formation and effectively sealing vascular injuries.
Dysfunction or deficiency in FXI can lead to bleeding disorders such as Hemophilia C although this is less common than Hemophilia A or B. Interestingly elevated levels of FXI are sometimes associated with an increased risk of thrombotic conditions such as deep vein thrombosis or stroke. In these conditions FXI interacts with other coagulation factors like Factor IX and Factor XII highlighting its dual role in both bleeding and clotting disorders. Understanding FXI's role provides insight into therapeutic targets for managing these conditions.