Abcam, ab108872, Rat IL-10 ELISA Kit

Size: 1 x 96Tests
Rat IL-10 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Rat IL-10 in Rat in Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Cell culture supernatant,
Reacts with:Rat,
Assay type:Sandwich (quantitative),
Sensitivity:= 45 pg/mL,
Range:0.063 - 4 ng/mL,
Assay time:4h 30m,
Assay Platform:Microplate

Product details:
Abcam's IL-10 Rat
in vitro
ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-10 in cell culture supernatants.
An IL-10 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an IL-10 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-10 captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols