Abcam, ab108910, Mouse TNF alpha ELISA Kit

Size: 1 x 96Tests
Mouse TNF alpha ELISA Kit is a sandwich ELISA designed to quantify Mouse TNF alpha with a sensitivity of 5.1 pg/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 31.25 - 2000 pg/mL
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Serum, Cell culture supernatant, Tissue, Cell Lysate,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 5.1 pg/mL,
Range:31.25 - 2000 pg/mL,
Assay time:5h,
Assay Platform:Microplate

Product details:
Mouse TNF alpha ELISA Kit ab108910 is a sandwich ELISA to measure Mouse TNF alpha in serum, plasma, cell culture supernatant, cell lysate, tissue with a sensitivity of 5.1 pg/ml.
How the assay works
A TNF alpha specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a TNF alpha specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of TNF alpha captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Mouse TNF alpha ELISA Kit ab108910 protocol summary
1. Add standard or sample to appropriate wells. Incubate at room temperature
2. Wash and add prepared biotin antibody to each well. Incubate at room temperature.
3. Wash and add prepared Streptavidin-Peroxidase Conjugate. Incubate at room temperature
4. Add Chromogen Substrate to each well. Incubate at room temperature
5. Add Stop Solution to each well. Read at 450 nm immediately.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols