Abcam, ab110290, Anti-ACAT1 antibody [9H10AB4]

Size: 100µg
Mouse Monoclonal ACAT1 antibody. Suitable for ICC, IP, Flow Cyt, IHC-P and reacts with Human samples. Cited in 2 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:9H10AB4,
Isotype:IgG2a,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, IP, ICC, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Product details:
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Properties and Storage Information:
Form-Liquid, Purification technique-Precipitation Ammonium Sulphate, Purification notes-Purity near homogeneity as judge by SDS-PAGE. The antibody was produced in-vitro using hybridomas grown in serum-free medium and then purified by biochemical fractionation., Storage buffer-pH: 7.5Preservative: 0.02% Sodium azideConstituents: HEPES buffered saline, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ACAT1 also known as acyl-CoA:cholesterol acyltransferase 1 is an enzyme that catalyzes the formation of cholesteryl esters from cholesterol and long-chain fatty acyl-CoA. It has a molecular mass of approximately 50 kDa. ACAT1 is present in the endoplasmic reticulum membrane and is expressed widely including in liver adrenal glands and macrophages. The enzyme's activity is significant in lipid metabolism storing cholesterol as cholesteryl esters.
Biological function summary
ACAT1 plays a central role in cellular cholesterol homeostasis and in preventing cholesterol-induced cytotoxicity by converting free cholesterol into inert cholesteryl esters for storage. It functions as a homotetramer suggesting that it works as a part of a larger protein complex. ACAT1 influences lipid accumulation within cells and mediates the regulation of cholesterol levels which is vital for maintaining membrane integrity and cellular function.
Pathways
ACAT1 participates in the cholesterol metabolism pathway and the lipid biosynthesis pathway. It interacts with proteins such as LDLR (low-density lipoprotein receptor) by controlling the availability of free cholesterol within cells. These pathways are critical in maintaining lipid balance and influencing lipoprotein metabolism. ACAT1's enzymatic activity impacts how cells manage cholesterol influx and storage linking to broader lipid regulation networks.
ACAT1 is associated with atherosclerosis and Alzheimer's disease. The conversion of cholesterol into cholesteryl esters affects the development of atherosclerotic plaques contributing to cardiovascular disease risk. In Alzheimer's disease ACAT1 may influence the accumulation of amyloid-beta a protein linked to the pathology of the disease. The protein APP (amyloid precursor protein) connects to ACAT1 as disturbances in cholesterol metabolism can affect amyloid-beta production linking this enzyme to neurodegenerative conditions.