Abcam, ab116029, Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa)

Size: 500µL
5-245 kDa prestained protein ladder (ab116029) is a ready-to-use, three-color protein standard with 13 pre-stained proteins ranging from molecular weights 5 to 245 kDa. - Suitable for western blot (WB) and SDS-PAGE - Suitable for Tris Glycine (4-12%) SDS buffer, Bis-Tris (4-12%) MOPS buffer and Bis-Tris (4-12%) MES buffer - Part of our Lab essentials range western blot workflow
Key facts
Applications:SDS-PAGE, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Form:LiquidSee storage information,
Storage buffer:pH: 7.5Constituents: 15% Glycerol (glycerin, glycerine), 2% Sodium lauryl sulfate, 0.44% Tris citrate/phosphate, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Urea

Product details:
Prestained Protein Ladder ab116029 is a three-color protein standard with 13 pre-stained proteins covering a wide range molecular weights from 3.5 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).
This product is also sold by Expedeon, an Abcam company as RunBlue™ TriColor Prestained Protein Ladder: NXA6050. This product was previously called Prism Ultra Protein Ladder (3.5 - 245 kDa).
This prestained protein ladder is designed for monitoring protein separation during SDS polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading.
Key product features:
Broad range:
3.5-245 kDa
Ready-to-use:
Supplied in a loading buffer for direct loading on gels.
Easy to identify:
Includes green ~25 kDa and red ~75kDa reference bands.
Sharp bands
Recommended loading:
~1.5 - 2.5μl
Review other protein ladders in the
unstained and prestained protein ladder guide

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C