Abcam, ab117136, Methylated DNA Immunoprecipitation (MeDIP) Kit - Tissue

Size: 24Test / 48Test
DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes.
Key facts
Assay time:3h 30m,
Sample types:Tissue,
Reacts with:Mouse, Human

Product details:
DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes. It has been demonstrated that DNA methylation plays an important role in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases, and that alterations in DNA methylation patterns are associated with cancer.
Methylated DNA Immunoprecipitation (MeDIP) ChIP Kit (ab117136) enables the user to enrich
methylated DNA directly from various mammalian tissues
by using an antibody specific to methyl cytosine (5-mC) to immunoprecipitate methylated genomic DNA
(MeDIP)
. The enriched methylated fractions can then be used in various downstream applications including qualitative and quantitative PCR, as well as southern blot and DNA microarray.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C