Abcam, ab118787, Anti-INO80 antibody

Size: 100µg
Rabbit Polyclonal INO80 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 16 publications. Immunogen corresponding to Synthetic Peptide within Human INO80 aa 1500 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human INO80 aa 1500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q9ULG1

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab118787 was affinity purified using an epitope specific to INO80 immobilized on solid support., Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: 99% Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
INO80 also known as INO80 complex subunit or INO80 ATPase is a chromatin remodeling enzyme weighing approximately 180 kDa. It is located within the nucleus of cells and is found across various tissues. INO80 plays an important mechanical role in modifying the structure of chromatin by sliding and ejecting nucleosomes enabling DNA accessibility for replication repair recombination and transcription processes.
Biological function summary
The INO80 complex affects gene expression and DNA stability. The INO80 protein forms part of the larger INO80 chromatin remodeling complex which includes several other subunits. This complex helps regulate chromatin structure by repositioning nucleosomes and altering histone-DNA interactions. These actions support the maintenance of genome integrity and facilitate accurate gene expression by modifying the accessibility of DNA to transcription factors and the transcription machinery.
Pathways
The INO80 complex impacts critical pathways for DNA repair and replication such as the homologous recombination repair pathway and the cell cycle checkpoint pathways. The homologous recombination repair is essential for fixing double-strand breaks in DNA and involves related proteins such as RAD51. Moreover INO80 interacts with proteins like RuvB-like helicases which assist in the structural rearrangement of chromatin during these processes.
Malfunction of the INO80 complex relates to cancer and neurodegenerative diseases. Aberrant functions of INO80 can lead to genomic instability and compromised DNA repair mechanisms contributing to tumorigenesis. In cancer its interaction with proteins such as ATR influences cellular responses to DNA damage. Additionally disruptions in the normal functioning of chromatin remodeling proteins including INO80 have implications in neurodegenerative diseases where INO80’s role in maintaining chromatin structure can impact neuronal survival and health.