Abcam, ab213861, Mouse DMP-1 ELISA Kit

Size: 1 x 96Tests
Mouse DMP-1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse DMP-1 in Mouse in Cell Culture Media, Biofluids samples.
Key facts
Detection method:Colorimetric,
Sample types:Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:< 10 pg/mL,
Range:156 - 10000 pg/mL,
Assay time:3h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
The Mouse DMP-1 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213861) is designed for the quantitative detection of Mouse DMP-1 in cell culture supernatants, serum and plasma (heparin, EDTA).
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from rat specific for DMP-1 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: L17-Y503) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for DMP-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse DMP-1 amount of sample captured in plate.
Dentin matrix acidic phosphoprotein 1 is a protein that in humans is encoded by the DMP1 gene. It belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family of secreted phosphoproteins. This gene is mapped to 4q22.1. This protein, which is critical for proper mineralization of bone and dentin, is present in diverse cells of bone and tooth tissues. In undifferentiated osteoblasts, DMP-1 is primarily a nuclear protein that regulates the expression of osteoblast-specific genes. During osteoblast maturation, the protein becomes phosphorylated and is exported to the extracellular matrix, where it orchestrates mineralized matrix formation. Mutations in the gene are known to cause autosomal recessive hypophosphatemia, a disease that manifests as rickets and osteomalacia. The gene structure is conserved in mammals.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein DMP1 also known as Dentin Matrix Protein 1 is part of the SIBLING (Small Integrin-Binding Ligand N-linked Glycoprotein) family and plays an important role in bone and tooth mineralization. It is encoded by the DMP1 gene and has a molecular weight of around 52 kDa. DMP1 localizes mainly to the extracellular matrix of bone and dentin and is highly expressed in osteoblasts odontoblasts and hypertrophic chondrocytes. This protein regulates the formation of hydroxyapatite a critical mineral component in the formation of hard tissues.
Biological function summary
The protein DMP1 is essential for phosphate homeostasis skeletal development and mineralization processes. It acts as a nucleator in the deposition of calcium phosphate minerals. As part of a protein complex DMP1 interacts with other extracellular matrix proteins influencing their functions. DMP1 plays an important role in the maturation of bone matrix and dentin effectively mediating the transformation of mesenchymal stem cells into osteoblasts and odontoblasts. It is also implicated in the regulation of gene expression linked to mineralization.
Pathways
DMP1 is closely involved in the bone mineralization and FGF (Fibroblast Growth Factor) signaling pathways. These pathways are important for maintaining bone density and controlling phosphate metabolism. DMP1 interacts with other proteins like PHEX (Phosphate Regulating Endopeptidase Homolog X-Linked) and MEPE (Matrix Extracellular Phosphoglycoprotein) within these pathways to facilitate proper mineralization of bone and teeth. Furthermore DMP1 functions alongside other SIBLING family proteins to modulate mineralization through their collective roles in the matrix.
DMP1 mutations or dysfunctions lead to conditions such as autosomal recessive hypophosphatemic rickets and osteomalacia. These conditions result from defective phosphate metabolism affecting skeletal mineralization and causing brittle bones and dental anomalies. In these scenarios DMP1's relationship with PHEX becomes evident as both proteins work together to regulate phosphate levels. Disruption in their function can significantly alter mineralization balance highlighting the necessity of DMP1 in maintaining skeletal and dental health.