Abcam, ab216263, Anti-Intron-encoded endonuclease I-SceI antibody

Size: 100µL
Rabbit Polyclonal Intron-encoded endonuclease I-SceI antibody. Suitable for WB, ICC/IF and reacts with Saccharomyces cerevisiae samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C SCEI.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Saccharomyces cerevisiae,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C SCEI.P03882

Properties and Storage Information:
Form-Liquid, Purity-Whole antiserum, Storage buffer-Preservative: 0.05% Sodium azide, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Intron-encoded endonuclease I-SceI also known as endonuclease I-SceI or I-SceI endonuclease is a precise and highly specific endonuclease. It recognizes and cleaves an 18-base pair sequence. This sequence is rare resulting in the enzyme being used as a molecular tool for genome editing. I-SceI is encoded by a group I intron within the mitochondrial DNA of yeast and possesses a molecular mass of approximately 30 kDa. The enzyme expression is primarily observed in eukaryotic organisms often associated with mitochondrial genetic material.
Biological function summary
I-SceI facilitates homologous recombination and DNA repair by introducing double-strand breaks at specific genetic locations. Through this action it participates in gene conversion processes allowing for the correction of genetic sequences. It does not operate as a part of a larger protein complex; rather its function centers on individual action and recognition of its target sites. Biological effectiveness makes it an attractive candidate for genomic studies and manipulations.
Pathways
I-SceI contributes significantly to DNA repair pathways particularly homologous recombination. This pathway is important for maintaining genomic stability. I-SceI cooperates with proteins such as RAD51 which facilitates strand exchange during the DNA repair process. In another pathway I-SceI-induced breaks can spur the activation of the single-strand annealing pathway when homologous recombination is impaired showcasing its versatile role in DNA repair mechanisms.
I-SceI's role in DNA repair connects it to cancer research. Impaired DNA repair mechanisms can lead to genomic instability a contributing factor to tumorigenesis. By enabling targeted gene corrections I-SceI may present therapeutic potential in correcting cancer-causing mutations. Additionally its interaction with the RAD51 protein implicates it in conditions where DNA repair is compromised such as certain genetic disorders with defective recombination.