Abcam, ab217055, Anti-AP1S1 antibody

Size: 100µg
Rabbit Polyclonal AP1S1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human AP1S1 aa 100 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human AP1S1 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.P61966

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab217055 was affinity purified using an epitope specific to AP1S1 immobilized on solid support., Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: 99% Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
AP1S1 also known as Adaptor Protein complex 1 Sigma 1 is an integral component of the AP-1 adaptor complex. This complex is involved in the sorting and trafficking of transmembrane proteins. AP1S1 has a molecular mass of approximately 19 kDa and is universally expressed in various tissues including the liver and brain. Its main function involves the interaction with clathrin aiding in the selection of cargo proteins for transport between the trans-Golgi network and endosomes.
Biological function summary
AP1S1 plays a significant role in vesicular trafficking processes. It participates as part of the AP-1 adaptor protein complex which is responsible for mediating sorting signals in coated vesicles. Through this participation AP1S1 influences the efficiency and specificity of intracellular transport. The AP-1 complex also interacts with phosphatidylinositol phosphates further regulating the vesicle formation and protein sorting mechanisms within cells. This protein's involvement is critical for maintaining cellular homeostasis and specific protein function.
Pathways
AP1S1 functions in pathways related to protein sorting and vesicular transport. It plays a role within the clathrin-mediated endocytosis pathway important for cellular receptor regulation and nutrient uptake. AP1S1 connects with other proteins such as clathrin and other adaptor proteins to form a network ensuring efficient cargo selection and transportation. This function ensures a streamlined process for the cellular uptake and regulation of essential molecules.
AP1S1 has links to various conditions through its role in protein trafficking. Mutations in AP1S1 associate with Mednik Syndrome a disorder characterized by neurological issues due to improper protein sorting. Another connection involves its association with hereditary spastic paraplegia where misregulation of protein trafficking results in neurological deterioration. In these contexts AP1S1 interacts with proteins like AP1B1 which similarly impacts vesicular transport and sorting pathways affecting overall cellular functionality.