Abcam, ab219044, Rat CXCL1 ELISA Kit (GRO alpha)

Size: 1 x 96Tests
Rat CXCL1 ELISA Kit (GRO alpha) is a Sandwich (quantitative) ELISA for the measurement of Rat CXCL1 (GRO alpha) in Rat in Cell/Tissue Extracts, Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Cell culture extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Rat,
Assay type:Sandwich (quantitative),
Sensitivity:= 4.39 pg/mL,
Range:18.75 - 1200 pg/mL,
Assay time:1h 45m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Rat CXCL1 ELISA Kit (GRO alpha) (ab219044) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CXCL1 (GRO alpha) protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat CXCL1 with 1.61 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
CXCL1 (previously called GRO1 oncogene) is a member of the CXC family of chemokines. CXCL1 expression is reported in lymphocytes such as macrophages and neutrophils as well as epithelial cells. It plays a role in neutrophil chemotaxis. CXCL1 is involved in spinal cord development via inhibition of oligodendrocyte precursor migration as well angiogenesis, arteriogenesis, inflammation, wound healing and tumorigenesis.
Rat CXCL2 and CXCL3 share 65% and 64% sequence homology with rat CXCL1, respectively. Additionally, mouse CXCL1 is 89% homologous with rat CXCL1.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CXCL1 also known as GRO alpha or KC in certain species is a chemokine with a molecular mass of around 7.8 kDa. This protein is primarily secreted by macrophages neutrophils and endothelial cells. It plays a critical role in the recruitment of neutrophils during inflammation and acts by binding to the CXCR2 receptor. CXCL1 is expressed in many tissues including lungs liver and skin where it regulates the immune response and facilitates tissue repair.
Biological function summary
CXCL1 functions as a significant mediator in inflammatory processes. It does not form a complex but acts independently to exert its effects. By attracting neutrophils to sites of injury or infection CXCL1 supports the body's defense mechanisms. This chemokine also influences angiogenesis contributing to the formation of new blood vessels which is essential in wound healing and tissue regeneration.
Pathways
CXCL1 plays a role in both the NF-kB signaling and MAPK signaling pathways. These pathways are pivotal for the regulation of immune and inflammatory responses. CXCL1 acts alongside proteins such as IL-8 another chemokine that also binds to the CXCR2 receptor. Through these pathways CXCL1 influences the activation and migration of immune cells facilitating a rapid response to inflammatory stimuli.
CXCL1 has a significant association with conditions such as chronic obstructive pulmonary disease (COPD) and rheumatoid arthritis. In COPD elevated levels of CXCL1 contribute to persistent inflammation and neutrophil infiltration. In rheumatoid arthritis CXCL1 participates in joint inflammation and damage interacting with proteins like TNF-alpha which amplifies inflammatory responses. Understanding the role of CXCL1 in these conditions could help guide the development of targeted therapies to mitigate inflammation and tissue damage.