Abcam, ab219915, Caspase-3/7, Caspase-8 and Caspase-9 Multiplex Activity Assay Kit (Fluorometric)

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Caspase-3/7, Caspase-8 and Caspase-9 Multiplex Activity Assay Kit (Fluorometric) ab219915 is used to monitor multiple caspase activation in live cells - 3 fluorogenic indicators for simultaneous monitoring of caspase3/7, caspase 8 and caspase 9 - Optimised for microplate reader - Up to 100 tests for each caspase
Key facts
Detection method:Fluorescent,
Sample types:Suspension cells, Adherent cells,
Assay type:Semi-quantitative,
Assay Platform:Microplate reader

Product details:
Caspase-3/7, Caspase-8 and Caspase-9 Multiplex Activity Assay Kit (Fluorometric) is designed to simultaneously monitor three key caspases involved in apoptosis: the initiator caspases caspase 8 and caspase 9, and the executioner Caspase 3.
How the assay works
The kit uses DEVD-ProRed™, IETD-R110 and LEHD-AMC as fluorogenic indicators for Caspase 3, caspase 8 and caspase 9 activity respectively. Upon caspase cleavage, three distinct fluorophores are released: ProRed™ (red fluorescence), R110 (green fluorescence) and AMC (blue fluorescence), which can be readily monitored in a single assay due to their spectral separation.
This product has been optimized for use in a microplate reader in a 96-well plate, providing enough reagent to perform 100 tests for each caspase.
Caspase-3/7, Caspase-8 and Caspase-9 protocol summary
- Grow and treat cells with test compounds to induce caspase activation
- Add caspase assay solution to cells
- Incubate at room temperature for 30-60 minutes
- Monitor fluorescence intensity at: Ex/Em = 535/620 nm (Caspase 3/7), Ex/Em = 490/525 nm (Caspase 8), Ex/Em = 370/450 nm (Caspase 9)
How other researchers are using this kit
ab219915 has been used in a variety of cancer cell lines including MCF-7 (breast cancer) cell line (1), U87MG (human glioblastoma) and MES-SA (human uterine sarcoma) cell lines (2).
References: 1 - T. Kumkoon et al. 2023; 2 - Yu Hsia et al. 2023.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C