Abcam, ab220507, HRP Anti-BrdU antibody [BU1/75 (ICR1)]

Size: 100µL
Rat Monoclonal BrdU antibody - conjugated to HRP. Suitable for IHC-P and reacts with Chemical samples. Cited in 2 publications.
Key facts
Host species:Rat,
Clonality:Monoclonal,
Clone number:BU1/75 (ICR1),
Isotype:IgG2a,
Conjugation:HRP,
Carrier free:No,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:This antibody reacts with BrdU in single stranded DNA, BrdU attached to a protein carrier or free BrdU. It detects nucleated cells in S-Phase which have had BrdU incorporated into their DNA. Also reacts with chlorodeoxyuridine but with reduced staining. The antibody does not react with thymidine. It has been reported in the literature that this antibody clone cross-reacts with Edu (PMID: 23272138) and some customers reported that it cross reacts with IdU.

Product details:
The antibody recognises single stranded DNA so the DNA needs to be unraveled first. This can be done with DNAse, although this doesn't give the best results. Depending on the assay, acid denaturation with 2M HCL or heat denaturation are the most successful. Please note this step is critical in any assay with this antibody and is the area that should be modified to optimise results. Detailed BrdU protocol is available

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.1% Proclin 300 SolutionConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark