Abcam, ab220776, Anti-SPDEF/PSE antibody

Size: 100µL
Rabbit Polyclonal SPDEF/PSE antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SPDEF aa 50-200.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human SPDEF aa 50-200. The exact immunogen used to generate this antibody is proprietary information.O95238

Product details:
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SPDEF also known as SAM pointed domain-containing ETS transcription factor is a protein with a mass of approximately 42 kDa. It functions as a transcription factor primarily regulating the expression of genes involved in cellular differentiation and proliferation. SPDEF is expressed mainly in epithelial tissues notably in the prostate lung and gastrointestinal tract. This expression pattern highlights its role in maintaining proper tissue homeostasis and function.
Biological function summary
SPDEF facilitates the differentiation of epithelial cells by influencing the transcription of lineage-specific genes. It interacts with other transcription factors and co-factors forming complexes to modulate gene expression with precision. Its involvement in mucin production defines a protective mechanism in epithelial lining contributing to barrier integrity. SPDEF's role in differentiation makes it an important player in epithelial tissue maintenance and function.
Pathways
SPDEF participates actively in the Wnt signaling pathway and the PI3K/AKT pathway. In the Wnt pathway it modulates the gene expression program to influence cell fate decisions often in collaboration with β-catenin. Regarding the PI3K/AKT pathway its role impacts cell survival and growth. SPDEF's interactions with proteins like FOXA1 and AR (Androgen Receptor) through these pathways draw a complex map of gene regulatory networks essential for cell function.
SPDEF is linked to prostate cancer and asthma. In prostate cancer aberrant SPDEF activity or expression influences tumor progression and metastasis often interacting with proteins such as AR. Conversely its role in asthma involves the regulation of mucin genes making it significant in airway hyperresponsiveness and remodeling. The connection between SPDEF and these diseases illuminates the overarching role of this transcription factor in health and disease.