Abcam, ab221839, Human MMP13 ELISA Kit

Size: 1 x 96Tests
Human MMP13 ELISA Kit is a sandwich ELISA designed to quantify Human MMP13 with a sensitivity of 5.7 pg/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 39 - 2500 pg/mL
Key facts
Detection method:Colorimetric,
Sample types:Saliva, Urine, Cell culture media, Serum,
Reacts with:Cow, Human, Pig,
Assay type:Sandwich (quantitative),
Sensitivity:= 5.7 pg/mL,
Range:39 - 2500 pg/mL,
Assay time:2h 30m,
Assay Platform:Microplate (12 x 8 well strips)

Product details:
Human MMP13 ELISA Kit ab221839 is a rapid single-wash 90-min Sandwich ELISA to measure Human MMP13 in . This SimpleStep sensitivity is 5.7 pg/mL.
How the assay works
Human MMP13 SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human MMP13 ELISA Kit ab221839 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-MMP13 antibody [EPR19120-143] - BSA and Azide free (Capture)
ab242921
- Anti-MMP13 antibody [EPR19120-115] - BSA and Azide free (Detector)
ab242921
Human MMP-13, matrix metallopeptidase 13, also known as Collagenase 3 is a secreted protein encoded by the gene MMP13. MMP-13 belongs to the matrix metalloproteinase family, which are calcium dependent endopeptidases. MMP-13 degrades type I, II, and III collagen. The MMP protein-13 is expressed as an inactive pro-form composed of an 83-amino acid pro-peptide, an amino acid catalytic domain, and hemopexin-like domain. Dissociation of a zinc ion from the cysteine-switch motif releases the activation-peptide, thus activating the enzyme. MMP-13 is involved in various processes, such as, embryonic development, ossification, wound healing, and tissue remolding.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Matrix metallopeptidase 13 (MMP-13) also called collagenase 3 is an enzyme with an approximate molecular mass of 54 kDa. It plays a significant role in the breakdown of extracellular matrix components particularly collagen. MMP-13 is expressed in several tissues including cartilage skin and bone. Its expression sees an increase during tissue remodeling and in pathological conditions. MMP-13 also participates in processes like wound healing and embryonic development making it a focal point for understanding tissue dynamics.
Biological function summary
Matrix metallopeptidase 13 contributes extensively to the degradation of collagen type II a major component of cartilage. As a zinc-dependent endopeptidase MMP-13 is part of the MMP family which facilitates the remodeling of the extracellular matrix. MMP-13 is involved in the proteolytic cascade working in conjunction with other MMPs and elastase to mediate tissue repair and turnover. It does not form complexes but acts in concert with other enzymes to execute its physiological functions effectively.
Pathways
Matrix metallopeptidase 13 significance is most noted in the cartilage degradation pathway. It interacts with other MMPs such as MMP-1 and MMP-9 to efficiently break down extracellular matrix components. These interactions highlight its role in the regulation of matrix metalloproteinase activity within the connective tissue degradation pathway. These pathways are key during normal connective tissue remodeling and in pathological processes illustrating the essential roles of MMPs in maintaining tissue homeostasis.
Matrix metallopeptidase 13 has strong associations with osteoarthritis and rheumatoid arthritis. It contributes to cartilage destruction intensifying the progression of these disorders due to its potent collagenolytic activity. Other proteins like MMP-9 and MMP-14 are also involved in these processes potentially amplifying tissue damage in affected joints. Understanding how MMP-13 and related proteins drive these diseases offers potential therapeutic targets for slowing disease progression and enhancing joint health.