Abcam, ab222922, Anti-PCGF3+PCGF5 antibody [EPR19475] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal PCGF3 antibody. Carrier free. Suitable for WB and reacts with Rat, Human, Mouse samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR19475,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Rat, Mouse,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab222922 is the carrier-free version of
ab201510
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4 Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The combined targets PCGF3 (Polycomb Group RING Finger 3) and PCGF5 (Polycomb Group RING Finger 5) are involved in transcriptional regulation through PRC1 (Polycomb Repressive Complex 1). These proteins usually have a molecular mass of approximately 34 kDa and 42 kDa respectively. PCGF3 and PCGF5 act as part of the non-canonical PRC1 complexes which are important in modulating gene repression and chromatin structure. Researchers have identified their expression in various tissues indicating a possible widespread role across the organism.
Biological function summary
These targets function as transcriptional repressors by facilitating the ubiquitination of histone H2A an important step in chromatin remodeling and gene silencing. PCGF3 and PCGF5 are components of the non-canonical PRC1 complexes where they interact with RING1B to impart their regulatory effects effectively. Their roles in these complexes suggest significant involvement in maintaining the repression of developmental genes and preserving cellular identity throughout development.
Pathways
PCGF3 and PCGF5 are essential players in chromatin modification and gene regulation pathways. They participate in the Polycomb pathways a pivotal element in controlling gene expression during differentiation and development. Within these pathways PCGF3 and PCGF5 interact with other Polycomb group proteins such as RING1A and CBX proteins to regulate specific sets of genes vital for cell fate decisions and lineage commitment.
Aberrations in PCGF3 and PCGF5 expression or function have connections to cancer and developmental disorders. Dysregulation of these proteins can lead to inappropriate activation or repression of genes necessary for normal cell growth contributing to oncogenesis. In particular their interaction with RING1B has been implicated in tumorigenesis highlighting their potential as targets for cancer therapy. Additionally their role in development suggests that mutations or misregulation might affect processes leading to congenital anomalies.