Abcam, ab224445, Anti-SHMT1 antibody - N-terminal

Size: 100µL
Rabbit Polyclonal SHMT1 antibody. N-terminal. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SHMT1 aa 1-100.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human SHMT1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.P34896

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Serine hydroxymethyltransferase 1 (SHMT1) is an enzyme that facilitates the conversion of serine to glycine while simultaneously producing 510-methylenetetrahydrofolate. It's also known by some as cytoplasmic SHMT. The enzyme mass is approximately 53 kDa. SHMT1 is mainly expressed in the cytoplasm of cells though expression can vary across different tissues with significant levels observed in liver and placenta.
Biological function summary
Serine hydroxymethyltransferase 1 plays a significant role in one-carbon metabolism. Particularly it is important for the synthesis of nucleotides which are the building blocks of DNA and RNA. SHMT1 exists in a homotetrameric form implying four SHMT1 units form a single functional enzyme complex. This homotetramer configuration allows it to effectively mediate the important reactions in the cytoplasm contributing to the maintenance of cellular function and proliferation.
Pathways
SHMT1 serves a pivotal role in both the folate and methionine cycles. These pathways are essential for cellular methylation reactions and DNA synthesis. SHMT1 is closely linked to the folate pathway interacting with proteins like methylene tetrahydrofolate reductase (MTHFR) that further utilize 510-methylenetetrahydrofolate produced by SHMT1. Its participation in these pathways highlights its importance in maintaining nucleotide balance and cellular methylation status.
Alterations in SHMT1 levels have been linked to cancer and neural tube defects. In cancer abnormal SHMT1 expression is associated with increased cell proliferation due to its impact on nucleotide synthesis and methylation status. Additionally the interplay between SHMT1 and proteins like thymidylate synthase can influence chemotherapy efficacy. In cases of neural tube defects maternal SHMT1 function can affect embryonic folate metabolism interacting with other proteins such as cystathionine beta-synthase (CBS) impacting folate homeostasis and fetal development.