Abcam, ab225555, Anti-ALIX antibody

Size: 100µL
Rabbit Polyclonal ALIX antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human PDCD6IP aa 800 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IP, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human PDCD6IP aa 800 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q8WUM4

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 6.8 - 7.4Preservative: 0.09% Sodium azideConstituents: Tris buffered saline, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ALIX also known as PDCD6IP or ALIX G is an adapter protein involved in various cellular processes. It holds a molecular weight of approximately 95-100 kDa and is widely expressed in different tissue types including HEK 293 cells. ALIX plays a significant role as a component of the endosomal sorting complexes required for transport (ESCRT) machinery contributing to membrane dynamics and intracellular trafficking.
Biological function summary
ALIX acts as an important mediator in the regulation of cellular membrane remodeling. It associates with several protein complexes influencing the budding and fission of vesicles and endosomes. The protein also interacts with ubiquitin and other factors guiding protein sorting and maintaining cellular homeostasis. This adapter characteristic makes ALIX essential for recycling receptors and maintaining cell surface receptor expression levels.
Pathways
ALIX is involved in the ESCRT pathway and the apoptotic pathways. It works closely with proteins like TSG101 and CHMP4 to facilitate vesicle formation and dispatch impacting cellular processes like signaling and waste disposal. Furthermore ALIX regulates apoptosis by interacting with proteins such as ALG-2 and to some extent with apoptosis-related membranes to mediate programmed cell death.
ALIX has established links to cancer progression and neurodegenerative disorders. Its participation in membrane remodeling and protein sorting correlates with tumor development with evidence showing that altered expression affects oncogenic pathways. Additionally dysregulation of ALIX-associated pathways has implications in neurodegenerative diseases where ALIX and proteins such as those in the ESCRT complex could play a role in the degradation of misfolded proteins and endocytic dysfunctions contributing to disease pathology.