Abcam, ab225974, Recombinant Pollen allergen Phl p 5b protein (His tag)

Size: 100µg
Recombinant Pollen allergen Phl p 5b protein (His tag) is a Phleum pratense Full Length protein, in the 20 to 284 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.
Key facts
Purity:>90% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:Mass Spec, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:Q40963,
Animal free:No,
Carrier free:No,
Species:Phleum pratense,
Storage buffer:pH: 7.2 - 7.4Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Pollen allergen Phleum pratense 5b commonly known as Phl p 5b is a major allergen protein found in timothy grass pollen. Phl p 5b plays an important role in triggering allergic reactions and measures approximately 35 kDa in mass. It is highly expressed in the reproductive tissues of grasses especially during the pollination season. The protein has structural regions that allows it to interact with immune system components which leads to sensitization in atopic individuals.
Biological function summary
Phl p 5b acts as a potent stimulus for the immune system by inducing the production of specific IgE antibodies. This protein is not usually a part of a larger complex but operates in tandem with other pollen allergens to exacerbate allergic responses. The presence of Phl p 5b rapidly activates mast cells and basophils promoting histamine release and inflammation. The protein’s strong immunogenic properties can lead to various allergic symptoms like rhinitis and asthma exacerbations.
Pathways
The interaction between Phl p 5b and the immune system involves key pathways such as the IgE-mediated hypersensitivity pathway and the Th2 (T-helper 2) immune response pathway. These pathways are integral to the allergic response leading to the release of chemical mediators that cause allergic symptoms. Phl p 5b’s interaction with IgE is central to its function as this enhances the activation of cells involved in allergic inflammation like mast cells and basophils.
Phl p 5b is most directly linked to allergic rhinitis and asthma. These common allergic disorders arise from exaggerated immune responses to inhaled pollen where Phl p 5b plays a significant role. The protein’s ability to bind to IgE correlates with the severity of allergic reactions. Moreover its potent immunogenic nature links it to other allergenic proteins which leads to cross-reactivity and complicates allergy management in sensitive individuals.