Abcam, ab227820, Anti-Sp7 / Osterix antibody [EPR21034] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Sp7 / Osterix antibody. Carrier free. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 8 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR21034,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, WB, IHC-Fr, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Mouse and rat species are recommended based on the IHC result. We do not guarantee western blot for mouse and rat.We recommend using 1% SDS hot method to prepare lysate to improve signal intensity.Please refer to the Protocol booklet under the Product Protocol section for the lysate preparation protocol.

Product details:
ab227820 is the carrier-free version of
ab209484
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Sp7 also known as Osterix is a transcription factor critical for bone formation. As a protein it has a mass of approximately 45 kDa. Sp7/Osterix is mainly expressed in osteoblasts cells responsible for new bone synthesis. The protein binds to specific DNA sequences regulating gene expression necessary for osteoblast differentiation and activity.
Biological function summary
Sp7 or Osterix functions as an important regulator in the development of the skeletal system. It operates as part of transcriptional regulation but does not form a complex with other proteins. By activating genes involved in bone matrix composition Sp7 drives the differentiation of precursor cells into mature osteoblasts which is an essential process for normal bone growth and maintenance.
Pathways
Sp7 works within the ossification pathway which is essential for bone development and regeneration. It closely interacts with Runx2 another transcription factor that plays a significant role in the regulation of osteoblast differentiation. The activity of Sp7 in this pathway ensures proper expression of bone-specific proteins including those encoded by the Spp1 and Calm genes which contribute to mineralization and calcium homeostasis.
Mutations or dysregulation of Sp7/Osterix impact conditions like osteogenesis imperfecta and osteoporosis. In these diseases impaired or excessive bone formation can occur affecting skeletal integrity. The protein's functional relationship with Runx2 is important in these contexts as both proteins together determine the balance between bone formation and resorption which when disrupted leads to pathological skeletal changes.