Abcam, ab228525, Autophagy Marker (APG5L/ATG5, ATG16L1, ATG4B, ATG9A, Beclin 1, LC3B) Antibody Sampler Panel

Size: 1Kit
Autophagy Antibody Panel Sampler Panel (ab228525) contains multiple trial-sized versions of clones against APG5L/ATG5, ATG16L1, ATG4B, ATG9A, Beclin 1, and LC3B specifically selected for high performance in various applications.
Key facts
Reacts with:Mouse, Rat, Human,
Target:ATG5See target data,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Product details:
Autophagy Antibody Panel Sampler Panel (ab228525) contains multiple trial-sized versions of clones against APG5L/ATG5, ATG16L1, ATG4B, ATG9A, Beclin 1, and LC3B specifically selected for high performance in various applications. This panel contains 6 recombinant rabbit monoclonal antibodies, 5 of which are knock-out validated. They are provided as a sampler panel to allow you to easily evaluate each in your required applications.
For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.
Panel contains:
- Rabbit monoclonal [EPR1755(2)] to APG5L/ATG5 (20 μL)
ab108327
- Rabbit monoclonal [EPR15638] to ATG16L1 - N-terminal (20 μL)
ab187671
- Rabbit monoclonal [EPR6436(2)] to ATG4B (20 μL)
ab154843
- Rabbit monoclonal [EPR2450(2)] to ATG9A (20 μL)
ab108338
- Rabbit monoclonal [EPR19662] to Beclin 1 (20 μL)
ab207612
- Rabbit monoclonal [EPR18709] to LC3B (20 μL)
ab192890
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Use our intuitive search and select carrier-free or your label of choice. For bespoke conjugations or large volumes email bespoke@abcam.com.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Beclin 1 also known as ATG6 or VPS30 is a central player in the autophagy process. This protein has a molecular mass of approximately 60 kDa and is widely expressed in a variety of tissues. Beclin 1 forms a core component of the class III phosphatidylinositol 3-kinase (PI3K) complex and helps to initiate the formation of pre-autophagosomal structures. Associated proteins like APG5L/ATG5 ATG16L1 ATG9A LC3B and ATG4B also play important roles in the regulation of autophagy. Particularly LC3B serves as an important autophagy marker often used to monitor autophagosomes through autophagy antibodies in experimental settings.
Biological function summary
These proteins coordinate to regulate and execute autophagy a process that cleans up damaged cellular components and helps maintain cellular homeostasis. They assemble into a complex network that orchestrates the formation and maturation of autophagosomes effectively marking them for lysosomal degradation. Beclin 1 interacts with multiple proteins like Atg14 and UVRAG to form different PI3K complexes leading to nucleation of the autophagic membrane. ATG5 ATG12 and ATG16L1 work in a conjugation system that expands the autophagosome membrane while the proteolytic processing of LC3B is essential for autophagosome closure.
Pathways
The autophagy process plays a significant role in cellular pathways like nutrient sensing and stress response. The AMPK/mTOR pathway for example tightly regulates autophagy in response to cellular energy status. AMPK activates autophagy by inhibiting mTOR signaling allowing initiation of autophagosome formation. Proteins such as ATG9A are integral for the trafficking of autophagy-related vesicles affecting the recruitment and movement of membrane source for developing autophagosomes. The balance between PI3K-AKT signaling and mTOR is important for the coordination between cell growth and the autophagic activity mediated by these proteins.
Dysregulated autophagy pathways involving Beclin 1 and associated components like LC3B and ATG5 are linked to cancer and neurodegenerative diseases such as Alzheimer's. Alterations in autophagy can disrupt cellular homeostasis facilitating the development of malignancies through the evasion of cell death. In neurodegenerative contexts impaired autophagy leads to the accumulation of toxic proteins exacerbating disease progression. The interplay between Beclin 1 and proteins like Bcl-2 also links it to apoptosis suggesting that a delicate balance between autophagy and apoptosis is important in preventing these disorders.