Abcam, ab228596, Anti-IDH3A antibody

Size: 100µL
Rabbit Polyclonal IDH3A antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human, Zebrafish samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human, Zebrafish,
Applications:WB, ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial. The exact immunogen used to generate this antibody is proprietary information.P50213

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.01% Thimerosal (merthiolate)Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
IDH3A also known as Isocitrate Dehydrogenase 3 Alpha is an important part of the tricarboxylic acid (TCA) cycle also called the citric acid cycle. This protein weighs approximately 39 kDa and operates in the mitochondria present in various tissue types but especially active in energy-demanding tissues like muscle and brain. IDH3A enzymatically catalyzes the oxidative decarboxylation of isocitrate to alpha-ketoglutarate using NAD+ as a cofactor which is fundamental in energy production.
Biological function summary
IDH3A functions in the context of the IDH3 complex a heterotetramer that includes subunits IDH3B and IDH3G aside from the alpha subunit. It plays an important role in the TCA cycle's contribution to cellular respiration serving as a linchpin in metabolic pathways that generate ATP through oxidative phosphorylation. The complex formation is essential for maintaining the enzymatic activity and stability required for continuous energy supply.
Pathways
IDH3A is integral to both the TCA cycle and the associated respiratory electron transport chain which is important for ATP production. IDH3A works closely with proteins such as succinate dehydrogenase and cytochrome c in the TCA cycle and electron transport chain. This interaction facilitates the transfer of electrons and protons which is necessary for establishing the proton gradient used in ATP synthesis.
IDH3A's role in metabolic pathways links it to certain metabolic disorders and cancers. Mutations or dysregulations in IDH3A have shown connections to gliomas where proteins like IDH1 are also implicated sharing distorted metabolic pathways due to altered enzymatic function. Additionally disruptions in its normal activity might relate to metabolic syndromes where the balance in energy production and oxidative stress responses may become compromised.