Abcam, ab229980, Anti-PGLS antibody

Size: 50µL
Rabbit Polyclonal PGLS antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PGLS.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IP, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human PGLS.O95336

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.3Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PGLS also known as 6-phosphogluconolactonase operates in the pentose phosphate pathway by catalyzing the hydrolysis of 6-phosphogluconolactone into 6-phosphogluconate. This enzyme has an approximate mass of 27 kDa. PGLS expresses widely in various tissues but it is especially abundant in liver and adipose tissues where the pentose phosphate pathway activity is high.
Biological function summary
6-phosphogluconolactonase plays a role in cellular metabolism by ensuring the smooth progression of the pentose phosphate pathway. This pathway is important for the production of NADPH which acts as a reducing agent in several biosynthetic reactions. PGLS does not form part of a multiprotein complex but serves a singular enzymatic role essential for maintaining cellular redox state.
Pathways
The pentose phosphate pathway lies at the center of PGLS's function in its role of maintaining NADPH levels for reductive biosynthesis and protection against oxidative stress. In this pathway PGLS closely associates with glucose-6-phosphate dehydrogenase (G6PD) which provides the substrate for PGLS. Both play a sequential role in the oxidative branch of the pentose phosphate pathway influencing cellular processes such as fatty acid synthesis and ribose-5-phosphate production.
PGLS connects to the metabolic and oxidative balance within cells indirectly affecting conditions like cancer and metabolic disorders. Alterations in its activity or expression could lead to dysregulation of NADPH levels linking it with oxidative stress-related pathologies. PGLS's functional relation to G6PD could influence its involvement in diseases where altered glucose metabolism and the oxidative state are disturbed.