Abcam, ab231432, Anti-Blood Group Lewis a antibody [SPM 522]

Size: 100µL / 500µL / 1mL
Mouse Monoclonal Blood Group Lewis a antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:SPM 522,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A/G, Purification notes-Purified from TCS by Protein A/G., Storage buffer-pH: 7.6Preservative: 0.1% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Blood Group Lewis a also known as Le^a is a carbohydrate antigen expressed on the surface of red blood cells and many epithelial tissues. It is part of the Lewis blood group system and its structure includes fucosylated glycan chains. The mass of the antigen is not readily available due to its nature as a carbohydrate chain component rather than a protein. This antigen can be found on the mucosal surfaces and in secretions where it plays a role in determining the Lewis blood type.
Biological function summary
Blood Group Lewis a serves as a ligand for selectins which are cell adhesion molecules. These interactions are important for cell-cell communication and for immune response modulation. The Lewis a antigen is not a standalone protein and does not function as part of a larger protein complex. Its carbohydrate structure assists in the recognition and binding processes involving selectins during cell signaling.
Pathways
The role of Blood Group Lewis a connects with the glycosylation pathways that modify proteins and lipids by adding sugar moieties. It also interacts with proteins in the cell adhesion pathways like E and P-selectins during the inflammatory response. Fucosyltransferases are the key enzymes involved in synthesizing Lewis a structure through the addition of fucose units onto the precursor glycan substrates.
Alterations in the Lewis antigen expression including Lewis a associate with gastric cancer and certain types of inflammatory disorders. Abnormal levels of this antigen can affect the binding of Helicobacter pylori contributing to gastric tissue changes and cancer development. Additionally changes in Lewis a expression can influence the interaction with proteins involved in cell adhesion during inflammatory responses potentially affecting conditions like inflammatory bowel disease.