Abcam, ab231729, Anti-ATP4A antibody

Size: 100µg
Rabbit Polyclonal ATP4A antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 3 publications.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Purification notes-Followed by Protein A affinity chromatography., Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 55.77% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ATP4A also known as the gastric H+/K+ ATPase alpha subunit is an important enzyme in the gastric lining. It is a P-type ATPase with a molecular mass of approximately 100 kDa. ATP4A primarily expresses in the parietal cells of the stomach where it contributes to the secretion of hydrochloric acid. This alpha subunit forms a catalytic core that cooperates with the beta subunit to actively transport ions across the stomach cell membrane.
Biological function summary
ATP4A facilitates the acidification process necessary for digestion. It operates as part of the gastric proton pump complex. This complex exchanges K+ ions from the gastric lumen with H+ ions from within the parietal cell cytoplasm driving the generation of stomach acid. The enzyme's action is central to the maintenance of the acidic pH in the stomach which is important for proper protein digestion and absorption of various nutrients.
Pathways
ATP4A plays a major role in the gastric acid secretion pathway. This pathway includes interactions with the carbonic anhydrase enzyme which provides the necessary bicarbonate ions to initiate the acidification process. ATP4A also forms interactions with the Na+/K+ ATPase as a part of ion balance and energy supply ensuring that the ionic gradients are appropriately established for gastric functions.
ATP4A's function is directly related to conditions like peptic ulcer disease and gastric cancer. Hyperactivity of ATP4A can lead to excessive acid secretion contributing to peptic ulcers. Additionally its chronic overexpression may have links to gastric cancer through persistent mucosal damage. Research also explores its association with other proteins like the epidermal growth factor receptor (EGFR) which can influence gastric tissue proliferation and healing processes.