Abcam, ab233197, Anti-P4HA2 antibody

Size: 100µg
Rabbit Polyclonal P4HA2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human P4HA2 aa 200 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human P4HA2 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.O15460

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Purification notes-Antigen-specific affinity chromatography followed by Protein A affinity chromatography., Storage buffer-pH: 7.4Preservative: 0.011% Proclin 300Constituents: PBS, 55.77% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
P4HA2 also known as Prolyl 4-hydroxylase subunit alpha-2 is an enzyme with an approximate mass of 60 kDa. It plays a role in catalyzing the hydroxylation of proline residues in collagen strands which is essential for the proper formation of the collagen triple helix structure. P4HA2 can be found most abundantly in tissues that require strong extracellular matrices such as skin tendons and cartilage. Its expression in these tissues suggests a critical role in maintaining structural integrity.
Biological function summary
Prolyl 4-hydroxylase functions within a larger enzyme complex by working alongside other subunits to ensure efficient collagen biosynthesis. This complex modifies proline residues on collagen peptides ensuring structural stability and mechanical strength of the extracellular matrix. By contributing to collagen stability P4HA2 supports cellular functions related to tissue maintenance and repair. Its activity is important in physiological environments where collagen is abundant emphasizing its role in vertebrate development and organism physiology.
Pathways
Collagen biosynthesis and modification serve as significant pathways involving P4HA2. This enzyme contributes to pathways related to the post-translational modification of collagen which is essential for extracellular matrix organization and cellular function. P4HA2 interacts with proteins such as collagen type I and type III playing an important role in the maturation of collagen fibers and influencing tissue resilience and elasticity. Such interactions highlight its involvement in fundamental biological processes that sustain organismal structure and function.
P4HA2 has associations with fibrosis and certain cancers. During fibrotic progression dysregulation of collagen production often involves elevated P4HA2 levels driving excessive extracellular matrix deposition and tissue stiffening. This condition can connect P4HA2 to fibrosis-related proteins such as TGF-beta which regulate extracellular matrix production. In cancer P4HA2 may contribute to tumor microenvironment changes influencing tumor growth and metastasis through interactions with proteins like MMPs (matrix metalloproteinases). Understanding these connections not only helps in identifying potential therapeutic targets but also in elucidating the pathophysiology of collagen-related diseases.