Abcam, ab23418, Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A74]

Size: 100µg / 100µL
Mouse Monoclonal Heparan Sulfate Proteoglycan 2/Perlecan antibody. Suitable for IP, ELISA, WB, IHC-P, ICC/IF, AP, IHC-Fr and reacts with Human, Cow samples. Cited in 20 publications. Immunogen corresponding to Cell preparation containing Heparan Sulfate Proteoglycan 2/Perlecan protein.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:A74,
Isotype:IgG1,
Carrier free:No,
Reacts with:Cow, Human,
Applications:AP, WB, IHC-P, IP, ICC/IF, ELISA, IHC-FrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Epitope:Epitope is located in domain V of Perlecan.,
Specificity:ab23418 is highly specific for perlecan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin).

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-pH: 7.4Preservative: 0.097% Sodium azideConstituents: PBS, 2.9% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Heparan Sulfate Proteoglycan 2 also known as Perlecan is a large multidomain proteoglycan with a mass of over 500 kDa. It contains heparan sulfate chains that contribute to its role in the extracellular matrix. Perlecan is expressed in various tissues including cartilage basement membranes and vascular tissues. It interacts with other matrix proteins providing structural integrity and influencing cell behavior.
Biological function summary
Perlecan plays a critical role in cell adhesion proliferation and growth factor signaling. It is an important component of basement membranes and forms a structural complex with other matrix proteins. Its interaction with heparan sulfate chains enables binding with various ligands such as growth factors and cytokines optimizing their availability and functionality at the cell surface. This interaction is essential in tissue maintenance and repair processes.
Pathways
Perlecan participates in the regulation of the Wnt and Hedgehog signaling pathways. These pathways influence key developmental processes and dictate cellular differentiation and migration. Perlecan and its heparan sulfate chains modulate protein interactions within these pathways notably affecting proteins such as Dsh and Ctnnb1 which are essential in signal transduction.
Perlecan involvement has been linked with tumorigenesis and cardiovascular diseases. Aberrations in its structure or expression can alter cellular microenvironments impacting key processes like angiogenesis and metastasis. Furthermore its interaction with growth factors can facilitate the progression of diseases like cancer highlighting its connection with proteins such as VEGF and TGF-beta which play major roles in disease modulation.