Abcam, ab235036, Anti-MT antibody

Size: 100µL
Rabbit Polyclonal MT antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MCAT aa 1-150.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human MCAT aa 1-150. The exact immunogen used to generate this antibody is proprietary information.Q8IVS2

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purity >95%., Storage buffer-pH: 7.4Preservative: 0.03% Proclin 300Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The metallothioneins (MT) are a family of cysteine-rich proteins known for their ability to bind heavy metals through the thiol groups of their cysteine residues. With a low molecular weight of approximately 6-7 kDa these proteins play important roles in metal ion homeostasis and detoxification processes. MT proteins are expressed in various tissues throughout the body including the liver kidney and immune cells where they confer protection against metal toxicity and oxidative stress.
Biological function summary
Metallothioneins serve as important mediators for maintaining metal ion balance within cells. They do not work alone but are part of larger protein complexes that facilitate the storage and transport of essential metal ions like zinc and copper. Through metal ion binding MTs modulate the activity of metal-dependent enzymes and transcription factors impacting processes like cell proliferation and apoptosis.
Pathways
Metallothioneins intersect heavily with cellular metal regulation and oxidative stress response pathways. They closely interact with proteins such as zinc transporters and metalloproteins influencing the cellular uptake distribution and utilization of zinc and other metals. The oxidative stress response pathway which involves proteins like the antioxidant enzyme superoxide dismutase also sees the participation of MTs aiding in cellular defense against reactive oxygen species.
Metallothioneins have a notable connection with conditions such as neurodegenerative diseases and cancers. In Alzheimer's disease for example the abnormal regulation of metal ions contributes to pathology with MTs interacting with proteins like amyloid-beta. Similarly in various types of cancers the expression levels of MTs influence tumor growth and resistance to chemotherapy with an observed interaction with proteins such as p53 tumor suppressor. These links make metallothioneins a target of significant interest in both clinical research and therapeutic development.