Abcam, ab154404, Anti-Asporin antibody

Size: 25µL / 100µL
Rabbit Polyclonal Asporin antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human ASPN aa 100-250.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse, Rat,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human ASPN aa 100-250. The exact immunogen used to generate this antibody is proprietary information.Q9BXN1

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.01% Thimerosal (merthiolate)Constituents: PBS, 10% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Asporin also known as ASPN is a small leucine-rich proteoglycan. Asporin weighs about 39 kDa. It expresses mostly in cartilage with notable presence in fibrocartilage and intervertebral discs. Scientists have identified asporin as a regulator of extracellular matrix organization and a mediator in cellular processes such as cell adhesion and migration. In Singapore and other places research on this protein has gained significant attention due to its roles in tissue development and potential clinical implications.
Biological function summary
The protein functions in modulating the extracellular matrix and influences mineralization of tissues. It belongs to a family of small leucine-rich repeat proteins that typically form part of larger complexes. Asporin interacts with TGF-β (transforming growth factor-beta) inhibiting its signaling pathway. This interaction affects collagen fibrillogenesis and may influence tissue repair processes. The ability of asporin to bind calcium hints at its role in calcification processes as well.
Pathways
Asporin plays a significant role in the regulation of the TGF-β and BMP (bone morphogenetic protein) signaling pathways. These pathways are key in controlling cell growth and differentiation particularly in connective tissues. Through these pathways asporin interacts with proteins like collagen I and II as well as other matrix components. It’s involved in fine-tuning processes such as skeletal development and cartilage homeostasis.
Research reveals connections between asporin and osteoarthritis as well as intervertebral disc disease. Variants in asporin known as polymorphisms have been linked to susceptibility to osteoarthritis in certain populations. Its interaction with TGF-β impacts disease progression implicating other proteins such as matrix metalloproteinases in the extracellular matrix breakdown. Asporin's modulation of tissue mineralization also relates to intervertebral disc degeneration providing insights into potential therapeutic targets.