Abcam, ab219998, Anti-Myogenin antibody

Size: 100µL
Rabbit Polyclonal Myogenin antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MYOG aa 100 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human MYOG aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.P15173

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Myogenin also known as MYOG is a muscle-specific transcription factor that plays an important role in the regulation of muscle differentiation and development. It belongs to the MyoD family of basic helix-loop-helix proteins. Myogenin has a molecular weight of approximately 34 to 39 kDa. It is typically expressed in skeletal muscle tissues aiding in the transition from proliferating myoblasts to differentiated myotubes. Immunohistochemistry (IHC) methods often use myogenin antibodies like the 'myogenin anticuerpo' and 'F5D' to identify myogenin expression within tissue samples facilitating the understanding of muscle tissue organization and development.
Biological function summary
Myogenin functions in controlling the transcription of muscle-specific genes that are essential during myogenesis. This transcription factor forms heterodimers with E-proteins becoming part of DNA-binding complexes that activate the transcription of muscle-specific genes. Myogenin's presence is critical during muscle cell fusion and maturation signifying its importance in proper muscle tissue formation and maintenance. Myogenin staining is often used as a technique to study muscle differentiation in various experimental settings.
Pathways
Myogenin is integrated into the myogenic regulatory network where it activates genes involved in muscle fiber maturation. It participates in the MyoD regulatory pathway working alongside other myogenic regulatory factors like MyoD Myf5 and MRF4. These proteins create a coordinated network ensuring efficient muscle cell differentiation and growth. The cross-talk between myogenin and these factors highlights its role in the intricate web of gene regulation necessary for muscle development.
The altered expression or mutation of the myogenin gene has potential implications in muscular dystrophies and myopathies. For instance disruptions in myogenin activity can lead to impaired muscle repair and regeneration contributing to the pathology of conditions such as rhabdomyosarcoma. Within these contexts myogenin associates with other proteins like MyoD whose expression might also be altered further accentuating the disruption in normal muscle differentiation processes during disease states.