Abcam, ab68194, Anti-alpha Actinin/ACTN1 antibody [EP2527Y] - Loading Control

Size: 100µL / 1mL
Anti-alpha Actinin/ACTN1 antibody [EP2527Y] (ab68194) is a rabbit monoclonal antibody detecting alpha Actinin/ACTN1 in Western Blot, IP, ICC/IF . Suitable for Human, Mouse, Rat . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 10 publications - Trusted since 2008
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP2527Y,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, IP, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-alpha Actinin/ACTN1 antibody [EP2527Y] (ab68194) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of alpha Actinin/ACTN1?
Anti-alpha Actinin/ACTN1 [EP2527Y] (ab68194) specifically detects a band for alpha Actinin/ACTN1 (UniProt: P12814) at a molecular weight of 103kDa.
Trusted by the scientific community
Anti-alpha Actinin/ACTN1 [EP2527Y] (ab68194) was first used in a scientific publication in 2008 and has been cited over 10 times in peer-reviewed journals.
Trial sizes available!
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Specificity confirmed
The specificity of Anti-alpha Actinin/ACTN1 antibody [EP2527Y] (ab68194) has been confirmed by Western blot testing in ACTN1 Knockout HAP1 cells.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Alpha Actinin-1 also known as ACTN1 or Actn 1 is a cytoskeletal protein that cross-links actin filaments in muscle and non-muscle cells. With a mass of approximately 100 kDa it is an important component of cell structures such as Z-discs in skeletal muscle. Alpha Actinin is expressed in a range of tissues including skeletal cardiac and smooth muscle as well as in various non-muscle cells. Its main role is to organize the actin cytoskeleton providing structural support and enabling cell motility.
Biological function summary
Alpha Actinin-1 helps maintain the structural integrity of cells by anchoring actin filaments and connecting them to integrins or membrane proteins. It often functions as part of a protein complex interacting with other cytoskeletal components and signaling molecules to stabilize cellular structures. In muscle cells it blends with titin and other sarcomeric proteins to assist muscle contraction. Beyond structural roles it partakes in cellular signaling pathways by interacting with signaling proteins and influencing cellular responses to external stimuli.
Pathways
Alpha Actinin-1 is involved in the regulation of the actin cytoskeleton pathway as well as the integrin signaling pathway. In these pathways it collaborates with proteins such as vinculin and paxillin modulating cell adhesion and movement. Its ability to interact with integrin-linked kinases suggests a role in transducing mechanical signals from the extracellular matrix to downstream signaling cascades.
Alpha Actinin-1 has been linked to a spectrum of muscular diseases and certain cancers. Mutations in Alpha Actinin-1 can contribute to hereditary diseases like congenital kidney disorders which affect actin filament organization in podocytes. In oncology altered expression levels of Alpha Actinin-1 can affect cell proliferation impacting tumor growth and metastasis. Its interaction with other proteins like focal adhesion kinase (FAK) underlines its involvement in these pathological conditions influencing cellular adhesion and signal transduction.