Biolegend, 307821, APC/Fire™ 750 anti-human IgD Antibody, 25tests

IgD, a member of the immunoglobulin (Ig) family, is expressed in naïve B cells. It has 3 Ig-like domains and exists in a transmembrane and a soluble form. In general, IgD is not secreted and usually its expression is lost after the Ig isotype switch. After antigen binding, IgD signals through the CD79a/CD79b (Igα/Igβ) heterodimer, resulting in the activation of the B cell. (BioLegend Website)
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Native human IgD protein (full length)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.
Excitation Laser: Red Laser (633 nm)
Application Notes: This clone does not cross react with mouse tissues.
RRID: AB_3097320 (BioLegend Cat. No. 307821) AB_3097320 (BioLegend Cat. No. 307822)
Distribution: Naïve B cells
Function: Antigen binding, B cell activation
Cell Type: B cells
Biology Area: Immunology
Antigen References: Geisberger R, et al. 2006. Immunology. 118:429-37. Weller S, et al. 2005. Eur J Immunol. 35:2789-92. Brandtzaeg P & Johansen FE. 2005. Immunol Rev. 206:32-63.
Gene ID: 3495
UniProt: View information about IgD on UniProt.org
Clone: W18340F
Regulatory Status: RUO
Other Names: Ig delta chain C region
Isotype: Rat IgG2a, κ