Biolegend, 369356, Spark PLUS B550™ anti-human CD223 (LAG-3) Antibody, 100tests

CD223, also known as LAG-3, is a 70 kD type I transmembrane glycoprotein that is involved in T-cell signaling. Similar to CD4, CD223 binds MHC class II, but with a higher affinity. CD223 negatively regulates T-cell activation. It is expressed by activated T-cells and natural killer cells (NKs), as well as regulatory T-cells. It is transiently expressed on the surface of activated T-cells in acute conditions but high expression is maintained under tolerizing conditions. CD223 deficiency results in reduced tumor growth. CD223 and PD-1 can act in synergy and reverse exhausted phenotypes, improve tumor rejection, and control viral load.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human LAG-3 transfected cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark PLUS B550™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.* Spark PLUS B550™ has a maximum excitation of 516 nm and a maximum emission of 540 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: The staining of clone 11C3C65 cannot be blocked by clone 7H2C65, which is another anti-human CD223 (LAG-3) antibody.
RRID: AB_3675087 (BioLegend Cat. No. 369355) AB_3675087 (BioLegend Cat. No. 369356)
Structure: 70 kD transmembrane glycoprotein, Ig superfamily, highly homologous to CD4.
Distribution: Activated T-cells and natural killer cells (NKs) and regulatory T cells.
Function: Negatively regulates T-cell activation.
Ligand/Receptor: Binds MHC class II molecules.
Cell Type: Dendritic cells, NK cells, T cells, Tregs
Biology Area: Immunology, Inhibitory Molecules
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Castelli C, et al. 2014. Oncoimmunology. 3(11):e967146. 2. Poirier N, et al. 2011. Clin. Exp. Immunol. 164:265. 3. Juno JA, et al. 2015. Retrovirology. 12:17. 4. Casati C, et al. 2006. Cancer Res. 66:4450.
Gene ID: 3902
UniProt: View information about CD223 on UniProt.org
Clone: 11C3C65
Regulatory Status: RUO
Other Names: CD223, LAG-3, LAG3, lymphocyte-activation gene-3
Isotype: Mouse IgG1, κ